{"id":"W3092143815","doi":"10.1038/s41434-020-00198-7","title":"Engineered AAV8 capsid acquires heparin and AVB sepharose binding capacity but has altered in vivo transduction efficiency","year":2020,"lang":"en","type":"article","venue":"Gene Therapy","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Manitoba; Public Health Agency of Canada; University of Guelph","funders":"National Heart, Lung, and Blood Institute; Canadian Institutes of Health Research; Canadian Lung Association; Government of Canada","keywords":"Capsid; Transduction (biophysics); In vivo; Sepharose; Affinity chromatography; Biology; Heparan sulfate; Molecular biology; Recombinant DNA; Mutant; In vitro; Biochemistry; Heparin; Cell biology; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002833696,0.000241644,0.0002290634,0.00009087089,0.0001192171,0.0000588186,0.0002429094,0.0001545293,0.0001140291],"category_scores_gemma":[0.00002372043,0.0002382866,0.00008142777,0.0002376636,0.0001410722,0.00001019939,0.00004128585,0.0001586313,0.00000895525],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002799062,"about_ca_system_score_gemma":0.00008927836,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00007972243,"about_ca_topic_score_gemma":0.0001214435,"domain_scores_codex":[0.9984345,0.0001507015,0.0002479978,0.0005406768,0.0002035309,0.0004226089],"domain_scores_gemma":[0.9994341,0.00001245895,0.00004817781,0.0002854902,0.00004434137,0.0001754934],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001746305,0.00007460786,0.000427247,0.00001596648,0.00005272249,0.000004600668,0.0009177908,0.0004887062,0.9909362,0.000001608105,0.0002354653,0.005098801],"study_design_scores_gemma":[0.0020719,0.001782611,0.0004528445,0.000006085245,0.000004511684,0.00001132696,0.00007323711,0.0007659395,0.9910859,0.00001544147,0.003470161,0.0002600552],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9952006,0.002824048,0.0005040862,0.0008094038,0.0001157134,0.0004024008,0.00006703537,0.00003455863,0.00004214392],"genre_scores_gemma":[0.996276,0.002403219,0.0002619301,0.0004532476,0.0003723569,0.00004171821,0.00004841019,0.00004383471,0.00009933807],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004838746,"threshold_uncertainty_score":0.9717045,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04673529924870008,"score_gpt":0.2750163049989464,"score_spread":0.2282810057502463,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}