{"id":"W3092143815","doi":"10.1038/s41434-020-00198-7","title":"Engineered AAV8 capsid acquires heparin and AVB sepharose binding capacity but has altered in vivo transduction efficiency","year":2020,"lang":"en","type":"article","venue":"Gene Therapy","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Manitoba; Public Health Agency of Canada; University of Guelph","funders":"National Heart, Lung, and Blood Institute; Canadian Institutes of Health Research; Canadian Lung Association; Government of Canada","keywords":"Capsid; Transduction (biophysics); In vivo; Sepharose; Affinity chromatography; Biology; Heparan sulfate; Molecular biology; Recombinant DNA; Mutant; In vitro; Biochemistry; Heparin; Cell biology; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003216358,0.0005881346,0.000362621,0.0002787848,0.0001461007,0.0005422834,0.000424107,0.0005785989,0.001384023],"category_scores_gemma":[0.0002720812,0.0002597034,0.0002841907,0.0001702199,0.0004557823,0.0003438078,0.0002729524,0.0009011526,0.0007233935],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000473267,"about_ca_system_score_gemma":0.0002826548,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009238837,"about_ca_topic_score_gemma":0.0007147742,"domain_scores_codex":[0.999648,0.00005361895,0.00004004592,0.00006214475,0.0001216793,0.0000745905],"domain_scores_gemma":[0.9995674,0.00009151862,0.0001224654,0.0000750341,0.00005261786,0.00009087989],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002507209,0.00001536319,0.00004186825,0.000008128762,0.000002493025,0.00002091302,0.000005816432,0.00002753517,0.9994103,0.0001032747,0.00001310051,0.0003262788],"study_design_scores_gemma":[0.000005396091,0.00007062239,0.0005542406,0.000001571189,0.000005420095,0.0001589616,0.00000437558,0.0003696927,0.9977494,0.00002554721,0.001050815,0.000004035877],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9850026,0.0002999103,0.01188915,0.0001359297,0.00006937222,0.0000326669,0.0003386406,0.0004038964,0.001827777],"genre_scores_gemma":[0.9839972,0.0001701239,0.008528704,0.00005381502,0.00001168643,0.00002662998,0.0008360848,0.0002026206,0.006173172],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001384023,"threshold_uncertainty_score":0.004630029,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04673529924870008,"score_gpt":0.2750163049989464,"score_spread":0.2282810057502463,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}