{"id":"W3094188710","doi":"10.1101/2020.10.20.347138","title":"Kinetic and thermodynamic analysis defines roles for two metal ions in DNA polymerase specificity and catalysis","year":2020,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"DNA and Nucleic Acid Chemistry","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Institutes of Health; York University; HIV Research Trust; New York University Abu Dhabi; Welch Foundation","keywords":"Nucleotide; Chemistry; Enzyme; Metal ions in aqueous solution; Catalysis; DNA; Enzyme kinetics; Polymerase; DNA polymerase; Stereochemistry; Binding site; Cooperative binding; Metal; Crystallography; Active site; Biochemistry; Organic chemistry","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005028857,0.0005537706,0.0006060699,0.0003754037,0.0002636083,0.0009441017,0.0006659244,0.0005416062,0.0008643529],"category_scores_gemma":[0.001341765,0.0005716406,0.0003956897,0.0001878245,0.0007130339,0.0006125914,0.0003511128,0.0004957975,0.0001689992],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00101609,"about_ca_system_score_gemma":0.000523151,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001663735,"about_ca_topic_score_gemma":0.001173464,"domain_scores_codex":[0.9997254,0.00004411081,0.00002101518,0.0000703035,0.00009091141,0.00004826579],"domain_scores_gemma":[0.9997023,0.0001358744,0.00005278304,0.00002532497,0.00004857567,0.0000350624],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0009597074,0.0001900548,0.01072663,0.0002822145,0.00008786976,0.0002628313,0.0001251936,0.07971395,0.8849428,0.01264984,0.0001345385,0.009924388],"study_design_scores_gemma":[0.0001016973,0.0003444444,0.005863724,0.00001339878,0.00005417912,0.0001697515,0.00008152648,0.4665186,0.5212941,0.004298972,0.001190399,0.00006911517],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9799443,0.000639921,0.01698715,0.0001545951,0.00001957277,0.00003448885,0.00005591754,0.000104024,0.002059887],"genre_scores_gemma":[0.9978509,0.00009511139,0.001762316,0.00001002679,0.000001907522,0.00001386616,0.00002550884,0.000008615701,0.0002317605],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001663735,"threshold_uncertainty_score":0.00737232,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008515588901685259,"score_gpt":0.2186292679299324,"score_spread":0.2101136790282471,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}