{"id":"W3094598396","doi":"10.1002/adbi.202000118","title":"Cell‐Free Synthesis of Dopamine and Serotonin in Two Steps with Purified Enzymes","year":2020,"lang":"en","type":"article","venue":"Advanced Biosystems","topic":"Amino Acid Enzymes and Metabolism","field":"Biochemistry, Genetics and Molecular Biology","cited_by":11,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Giovanni Armenise-Harvard Foundation; Simons Foundation","keywords":"Aromatic L-amino acid decarboxylase; Tyrosine hydroxylase; Tryptophan hydroxylase; Aromatic amino acids; Biochemistry; Monoamine neurotransmitter; Serotonin; Dopamine; Enzyme; Tyrosine; Phenylalanine hydroxylase; Amino acid; Tryptophan; Phenylalanine; Biology; Chemistry; Endocrinology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008536361,0.0001679969,0.0002971784,0.00003201204,0.00002096356,0.000008501468,0.0001730932,0.00006274785,0.000003061536],"category_scores_gemma":[0.0000725706,0.0001341027,0.00003209907,0.0001504373,0.00006146492,0.000006904827,0.00009547479,0.0000615471,0.000001376192],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000004427251,"about_ca_system_score_gemma":0.00003159359,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005634955,"about_ca_topic_score_gemma":0.00008842902,"domain_scores_codex":[0.9990528,0.00004764065,0.0002468278,0.0003446824,0.00011058,0.0001975067],"domain_scores_gemma":[0.9993967,0.00001995033,0.0001268224,0.0003075301,0.00005221574,0.00009682013],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002266819,0.00003338465,0.003997588,0.0001816549,0.0000238096,0.000006334464,0.00007776339,0.0001327787,0.9924242,0.0001611,0.0001003451,0.002634355],"study_design_scores_gemma":[0.001498238,0.0002239062,0.0007518933,0.00005574676,0.00001771987,0.000009629522,0.0003924615,0.00005464096,0.9796974,0.000003996824,0.01710927,0.0001851068],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.991341,0.006647276,0.0004143258,0.0001830978,0.00005269139,0.000365346,0.00004031577,0.00001366439,0.0009422624],"genre_scores_gemma":[0.9976051,0.0003805052,0.001567176,0.0001006937,0.00009594637,0.00004946721,0.00001419354,0.00002476216,0.0001621118],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01700892,"threshold_uncertainty_score":0.5468549,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005581731041387821,"score_gpt":0.2165121296777374,"score_spread":0.2109303986363496,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}