{"id":"W3094598396","doi":"10.1002/adbi.202000118","title":"Cell‐Free Synthesis of Dopamine and Serotonin in Two Steps with Purified Enzymes","year":2020,"lang":"en","type":"article","venue":"Advanced Biosystems","topic":"Amino Acid Enzymes and Metabolism","field":"Biochemistry, Genetics and Molecular Biology","cited_by":11,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Giovanni Armenise-Harvard Foundation; Simons Foundation","keywords":"Aromatic L-amino acid decarboxylase; Tyrosine hydroxylase; Tryptophan hydroxylase; Aromatic amino acids; Biochemistry; Monoamine neurotransmitter; Serotonin; Dopamine; Enzyme; Tyrosine; Phenylalanine hydroxylase; Amino acid; Tryptophan; Phenylalanine; Biology; Chemistry; Endocrinology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002385108,0.0005211075,0.0005049476,0.0001689038,0.000181599,0.0004023377,0.0003703204,0.0004020935,0.001007244],"category_scores_gemma":[0.0001805719,0.0002587472,0.000291631,0.0002562905,0.0002095132,0.0002497089,0.000293431,0.000852617,0.001287171],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003157333,"about_ca_system_score_gemma":0.0003835447,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000685641,"about_ca_topic_score_gemma":0.001427097,"domain_scores_codex":[0.9997564,0.00002783845,0.00002480051,0.00006546914,0.00008757857,0.00003790436],"domain_scores_gemma":[0.999911,0.00002009185,0.00001300195,0.00002392115,0.00001566705,0.00001640007],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002948005,0.0000200692,0.0000517615,0.00006068205,0.000006320357,0.0000325299,0.000008605454,0.00006812283,0.9965843,0.0002688669,0.0001138063,0.002755455],"study_design_scores_gemma":[0.00001237795,0.0000823459,0.0003009771,0.000005488221,0.00001162232,0.0001504823,0.000005580558,0.0005394864,0.9909576,0.00007407114,0.007851265,0.000008764217],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5656826,0.008238485,0.4057426,0.0005310182,0.0005381648,0.0007045533,0.00324952,0.00230342,0.01300952],"genre_scores_gemma":[0.773103,0.00520969,0.1924336,0.000145407,0.00005167676,0.0004396523,0.007450871,0.0002421701,0.02092393],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001007244,"threshold_uncertainty_score":0.003369629,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005581731041387821,"score_gpt":0.2165121296777374,"score_spread":0.2109303986363496,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}