{"id":"W3097886583","doi":"10.3791/61693","title":"In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity","year":2020,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto; Lunenfeld-Tanenbaum Research Institute","funders":"Canadian Institutes of Health Research","keywords":"CRISPR; Genome editing; Cas9; Biology; Computational biology; Gene; Function (biology); Gene targeting; Trans-activating crRNA; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002512577,0.0001361423,0.0002267623,0.00009401321,0.00002028856,0.00002279195,0.00009423574,0.00007913257,0.00002626367],"category_scores_gemma":[0.00011645,0.0001347005,0.00005334218,0.00011605,0.00001194125,0.00001022958,0.00007897832,0.0001091777,0.000001420064],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000023472,"about_ca_system_score_gemma":0.00002513581,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000409961,"about_ca_topic_score_gemma":0.00001751579,"domain_scores_codex":[0.999002,0.0000724355,0.0003668722,0.0001908326,0.0001818181,0.0001859862],"domain_scores_gemma":[0.9995909,0.000009844717,0.00008742067,0.0000847527,0.00005910437,0.0001679351],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0008272087,0.00006145519,0.001101594,0.000009318301,0.0000304979,0.00003997591,0.0006761785,0.007876206,0.9882056,9.305484e-7,0.0003429793,0.0008280721],"study_design_scores_gemma":[0.002507575,0.00077802,0.000744487,0.00002061997,0.0000115122,0.00003090489,0.0003146519,0.003024332,0.9890549,0.000003184713,0.003357992,0.0001518385],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9741867,0.0009302701,0.02449801,0.0001305391,0.000101806,0.000130121,0.000003695137,0.000003072098,0.00001581018],"genre_scores_gemma":[0.994413,0.00009610714,0.004606823,0.0006561311,0.000149987,0.00000460156,0.000002425396,0.00002002326,0.0000509581],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02022626,"threshold_uncertainty_score":0.5492929,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0250328993123725,"score_gpt":0.4210401691104254,"score_spread":0.3960072697980528,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}