{"id":"W3101962389","doi":"10.1101/2020.11.12.380089","title":"Far-red fluorescent genetically encoded calcium ion indicators","year":2020,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":37,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; University of Alberta; Howard Hughes Medical Institute","keywords":"Fluorescence; Chemistry; Fluorescent protein; Affinities; Far-red; Calcium; Biophysics; Green fluorescent protein; Biochemistry; Biology; Red light; Gene; Optics; Physics; Botany","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002606483,0.0004043013,0.0002576676,0.0002162787,0.0001679785,0.0004909404,0.0003976858,0.0005960183,0.001537618],"category_scores_gemma":[0.0003166041,0.0001821185,0.0001915023,0.0001861052,0.0003465161,0.0003019088,0.0003278748,0.0007382636,0.0009049411],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007440964,"about_ca_system_score_gemma":0.0002756529,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009907938,"about_ca_topic_score_gemma":0.0009125846,"domain_scores_codex":[0.9998308,0.00002121252,0.00000791844,0.00004612792,0.00006169302,0.00003220162],"domain_scores_gemma":[0.9998325,0.00003282865,0.00003946895,0.00001809443,0.00003860083,0.0000384474],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003443143,0.000005520468,0.00008941368,0.00002229311,0.00000246572,0.00002198077,0.000009257581,0.0002467298,0.9981003,0.0003458159,0.0001357511,0.0009861079],"study_design_scores_gemma":[0.000007847882,0.00002786732,0.000418016,0.000004100825,0.000003058247,0.00007582964,0.000005168192,0.001098545,0.9941458,0.00005644513,0.004150575,0.000006788991],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9390558,0.001972097,0.04791322,0.0007320239,0.000117651,0.0001349707,0.001567606,0.000729395,0.007777309],"genre_scores_gemma":[0.9208491,0.001244182,0.06114986,0.0002241171,0.0000204745,0.0001328298,0.002195272,0.0001748326,0.01400932],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001537618,"threshold_uncertainty_score":0.00539875,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01317593134087045,"score_gpt":0.2454843076594005,"score_spread":0.23230837631853,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}