{"id":"W3103590274","doi":"10.1016/j.jim.2020.112931","title":"A method for expansion and retroviral transduction of mouse regulatory T cells","year":2020,"lang":"en","type":"article","venue":"Journal of Immunological Methods","topic":"T-cell and B-cell Immunology","field":"Immunology and Microbiology","cited_by":18,"is_retracted":false,"has_abstract":false,"ca_institutions":"BC Children's Hospital; University of British Columbia","funders":"Canadian Institutes of Health Research; Crohn's and Colitis Canada; Michael Smith Health Research BC; BC Children's Hospital","keywords":"Transgene; Transduction (biophysics); Adoptive cell transfer; FOXP3; Biology; CD28; Cell therapy; Immunology; Cell sorting; Cell biology; Viral vector; Genetically modified organism; Signal transduction; T cell; Genetically modified mouse; Colitis; Cancer research; Immune system; Stem cell; Flow cytometry; Gene; Genetics; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001471182,0.001965963,0.0008105641,0.003100176,0.001001774,0.0008256528,0.001888359,0.001310776,0.006127301],"category_scores_gemma":[0.0006975922,0.001149848,0.001485062,0.000993114,0.0005493899,0.0005752213,0.0006927426,0.004335782,0.005402591],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003704636,"about_ca_system_score_gemma":0.0006162557,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005471326,"about_ca_topic_score_gemma":0.001122699,"domain_scores_codex":[0.9986215,0.0002682024,0.00019544,0.0002413261,0.000443972,0.0002296197],"domain_scores_gemma":[0.9992983,0.0002426664,0.00009204473,0.0002077638,0.00007592826,0.00008322721],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001445814,0.0001695559,0.0001078194,0.0001440543,0.00001897487,0.000113815,0.00005999633,0.00009947197,0.9856281,0.001070425,0.0006571824,0.01178605],"study_design_scores_gemma":[0.000122676,0.0004507063,0.0008346724,0.00004327286,0.0001038423,0.0008247931,0.00001629837,0.0008498942,0.9649715,0.0003426017,0.03141141,0.00002832558],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.07818346,0.006156555,0.8830035,0.0006502475,0.001170284,0.006174654,0.006334213,0.005043653,0.01328328],"genre_scores_gemma":[0.2166824,0.008329274,0.7139704,0.0007063475,0.0004007809,0.009277079,0.01570812,0.001706425,0.0332192],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.006127301,"threshold_uncertainty_score":0.02049786,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03646791075130828,"score_gpt":0.3185867891024517,"score_spread":0.2821188783511434,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}