{"id":"W3106788650","doi":"10.1016/j.xpro.2020.100163","title":"Transduction of Primary AML Cells with Lentiviral Vector for In Vitro Study or In Vivo Engraftment","year":2020,"lang":"en","type":"article","venue":"STAR Protocols","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Canadian Institutes of Health Research; Otsuka Pharmaceutical; Princess Margaret Cancer Foundation; Takeda Pharmaceutical Company; Novartis","keywords":"Transduction (biophysics); Small hairpin RNA; Viral vector; In vivo; Biology; Stem cell; Bone marrow; Immune system; Myeloid leukemia; Cell biology; Haematopoiesis; Cancer research; Immunology; Cell culture; Gene; Genetics","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000101453,0.0001080223,0.0001812571,0.00003183074,0.00001156677,0.00000786918,0.0001062553,0.00004901395,0.00001815682],"category_scores_gemma":[0.0000070695,0.00008393582,0.00004137956,0.0000857816,0.00002236021,0.000005759941,0.00002200133,0.00005263411,8.147968e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001840042,"about_ca_system_score_gemma":0.0000915072,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002496788,"about_ca_topic_score_gemma":0.0001336169,"domain_scores_codex":[0.999243,0.00004955699,0.0002141264,0.0002632977,0.00008681806,0.000143257],"domain_scores_gemma":[0.9997453,0.000005481636,0.00005661673,0.0001270006,0.00003718514,0.00002843124],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.007809745,0.0004423033,0.0007238794,0.0001284515,0.00002690594,0.00000482127,0.0005426784,0.00002960423,0.9890848,6.928142e-7,0.0001322929,0.001073785],"study_design_scores_gemma":[0.002907387,0.006515812,0.0008916829,0.00003445072,0.000006940617,4.308556e-7,0.0004180342,0.00002496359,0.9872173,0.000004295679,0.001870605,0.0001080746],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9477211,0.000008918796,0.000332343,0.00004872344,0.0000126273,0.05176091,0.0000550616,0.00000400726,0.00005631071],"genre_scores_gemma":[0.9502668,0.00000246154,0.0006022402,0.00008032626,0.00005252161,0.04888174,0.00001986768,0.00001258687,0.00008150424],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.006073508,"threshold_uncertainty_score":0.3422803,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02934739556504282,"score_gpt":0.2843955043746264,"score_spread":0.2550481088095836,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}