{"id":"W3111841342","doi":"10.1016/j.jmb.2020.166759","title":"Anti-CRISPR AcrIE2 Binds the Type I-E CRISPR-Cas Complex But Does Not Block DNA Binding","year":2020,"lang":"en","type":"article","venue":"Journal of Molecular Biology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto","funders":"Canadian Institutes of Health Research","keywords":"CRISPR; DNA; Nuclease; Binding site; Cas9; Mutagenesis; Biology; Genetics; Computational biology; Chemistry; Gene; Mutation","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003832774,0.0006620008,0.0008171441,0.0003287036,0.0004687678,0.0009558321,0.0008360735,0.001001033,0.00966142],"category_scores_gemma":[0.0005789119,0.0005855977,0.0005383514,0.0002377844,0.0006000664,0.0004139598,0.0005879587,0.002095363,0.003990477],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007279595,"about_ca_system_score_gemma":0.0008206138,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002212449,"about_ca_topic_score_gemma":0.003639288,"domain_scores_codex":[0.9989815,0.00009490385,0.00007223279,0.0002507107,0.0003673472,0.0002331325],"domain_scores_gemma":[0.9993014,0.0002017506,0.00009554935,0.0001478228,0.00008409521,0.0001693015],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001071447,0.00003663212,0.0001629084,0.00004825093,0.00001416627,0.00004926002,0.000008605011,0.00007014581,0.9977902,0.0002227557,0.0003798935,0.001109944],"study_design_scores_gemma":[0.00001260802,0.00004921629,0.001582471,0.000003744376,0.000006839698,0.0001991789,0.00001111948,0.0007116347,0.9905332,0.00004061233,0.006841737,0.000007596212],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9241079,0.002335989,0.0396265,0.00115776,0.0007472427,0.0001766993,0.002572549,0.001983208,0.02729212],"genre_scores_gemma":[0.9467217,0.0005595236,0.01512907,0.0008457776,0.00004200627,0.0001099191,0.004510257,0.0004804723,0.03160124],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00966142,"threshold_uncertainty_score":0.03232062,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01828161858712773,"score_gpt":0.3326203089580139,"score_spread":0.3143386903708862,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}