{"id":"W3113474824","doi":"10.1261/rna.078220.120","title":"Fluorogenic aptamers resolve the flexibility of RNA junctions using orientation-dependent FRET","year":2020,"lang":"en","type":"article","venue":"RNA","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":31,"is_retracted":false,"has_abstract":true,"ca_institutions":"Simon Fraser University","funders":"U.S. Department of Energy; Argonne National Laboratory; Office of Research Infrastructure Programs, National Institutes of Health; National Center for Research Resources; National Institute of General Medical Sciences; Office of Science; National Institute of Diabetes and Digestive and Kidney Diseases; Lawrence Berkeley National Laboratory; National Heart, Lung, and Blood Institute; Natural Sciences and Engineering Research Council of Canada; National Cancer Institute; National Institutes of Health","keywords":"Biology; Aptamer; Förster resonance energy transfer; Computational biology; Flexibility (engineering); RNA; Orientation (vector space); Nucleic acid structure; Riboswitch; Biophysics; Genetics; Cell biology; Gene; Non-coding RNA; Fluorescence; Physics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003392727,0.000223846,0.0002066866,0.000274439,0.0001630589,0.0002923088,0.0004492289,0.0005397512,0.0007322298],"category_scores_gemma":[0.0005563486,0.0001485553,0.0001652557,0.0001689099,0.0003556584,0.0003258388,0.00023752,0.0006829944,0.00029501],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003001473,"about_ca_system_score_gemma":0.00007584507,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001744079,"about_ca_topic_score_gemma":0.0004777707,"domain_scores_codex":[0.9997378,0.00004798957,0.00001529283,0.00007472195,0.00008650028,0.00003773019],"domain_scores_gemma":[0.9997488,0.0001081829,0.00007674452,0.00002966886,0.00001728507,0.00001930652],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001448499,0.0000102434,0.00016918,0.00001255147,0.000002590752,0.00002203173,0.00001736946,0.0003436399,0.9971747,0.0003091692,0.00001988223,0.001904013],"study_design_scores_gemma":[0.000004132551,0.00003557544,0.001422626,0.000003532326,0.000003426711,0.0000903836,0.00002315262,0.004493023,0.9927049,0.0002882904,0.0009238653,0.000007073405],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8758472,0.001199555,0.1185013,0.0002106959,0.00004560505,0.00004847383,0.0002234215,0.0004803718,0.00344334],"genre_scores_gemma":[0.9679831,0.0004931421,0.03007702,0.0001072005,0.00001240852,0.00005322228,0.0001766831,0.00003539659,0.001061705],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007322298,"threshold_uncertainty_score":0.002449572,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03189153813429513,"score_gpt":0.3025518612481158,"score_spread":0.2706603231138207,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}