{"id":"W3114379815","doi":"10.1002/elps.202000167","title":"BirA*‐protein A fusion protein based BioEnhancer amplifies western blot immunosignal","year":2020,"lang":"en","type":"article","venue":"Electrophoresis","topic":"Biotin and Related Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"National Natural Science Foundation of China","keywords":"Western blot; Biotin; Fusion protein; DNA ligase; Blot; Target protein; Molecular biology; Dot blot; Biochemistry; Chemistry; Biology; Enzyme; DNA; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001080735,0.001108442,0.0006091377,0.0006936494,0.0003585405,0.000479191,0.0007691284,0.0006769054,0.002480044],"category_scores_gemma":[0.0004459874,0.0004551714,0.0005549057,0.000465194,0.0006291968,0.0006252165,0.0006556766,0.001604627,0.001534891],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004916727,"about_ca_system_score_gemma":0.0002562221,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003968186,"about_ca_topic_score_gemma":0.0006181534,"domain_scores_codex":[0.9990497,0.000213242,0.00006809911,0.0002777809,0.0002663309,0.0001248458],"domain_scores_gemma":[0.9996123,0.0001195185,0.00008008153,0.00006759368,0.00007430116,0.00004623101],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002810354,0.00001922545,0.00004769781,0.00004618293,0.000005253296,0.00001896765,0.00001172559,0.0000142024,0.9986643,0.000129096,0.00006951874,0.0009457876],"study_design_scores_gemma":[0.000004688987,0.00007480375,0.0005123248,0.000003866097,0.00001188721,0.0001939292,0.000007899174,0.0005844322,0.9949738,0.00006301246,0.003562446,0.000006857975],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.511398,0.005850337,0.4688651,0.0006457287,0.0004321144,0.0004153444,0.0009257362,0.003395986,0.008071725],"genre_scores_gemma":[0.6707933,0.00314082,0.3078608,0.0004080365,0.0000966037,0.0005243271,0.003865517,0.0004205323,0.01289009],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.002480044,"threshold_uncertainty_score":0.008296549,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009623111395060313,"score_gpt":0.2176171120236196,"score_spread":0.2079940006285593,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}