{"id":"W3118209894","doi":"10.1021/acschembio.0c00684","title":"Combining Rational Design and Continuous Evolution on Minimalist Proteins That Target the E-box DNA Site","year":2020,"lang":"en","type":"article","venue":"ACS Chemical Biology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":18,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Social Sciences and Humanities Research Council of Canada; Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; University of Toronto","keywords":"Leucine zipper; Zipper; DNA; Computational biology; bZIP domain; Enhancer; Phosphodiester bond; Biology; Transcription factor; Chemistry; Cell biology; Biochemistry; Computer science; RNA; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00009734378,0.0001233601,0.0001207122,0.0000093078,0.00005525355,0.00001303075,0.0001140872,0.0001492461,0.000009126206],"category_scores_gemma":[0.0001620139,0.00009292514,0.00003518121,0.00003976199,0.0001191555,0.00000172201,0.00008973801,0.0001090587,0.000006990172],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000007006297,"about_ca_system_score_gemma":0.00002046986,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000003401978,"about_ca_topic_score_gemma":4.884689e-7,"domain_scores_codex":[0.9993203,0.00005204147,0.0001188036,0.0002815774,0.00005071403,0.0001765816],"domain_scores_gemma":[0.9996939,0.00005178635,0.00003815139,0.0001226049,0.00002560405,0.00006793578],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001077859,0.0000139581,0.001015064,0.000005734218,0.00002155353,7.944999e-7,0.00005530937,0.00007312568,0.9970036,0.0005107335,0.0009310514,0.0002612703],"study_design_scores_gemma":[0.0004444432,0.0003516376,0.0005328011,0.000003884888,0.000009835832,0.00001015016,0.0000295865,0.0005335269,0.9889693,0.0002134255,0.008765409,0.0001359556],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9015036,0.0006901471,0.09498122,0.002264132,0.00005846162,0.0002856624,0.00001964915,0.00002068499,0.0001764393],"genre_scores_gemma":[0.9957896,0.00002471707,0.00273931,0.000995945,0.0002118171,0.00003474221,0.000169587,0.00001137982,0.00002292794],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.09428597,"threshold_uncertainty_score":0.3789377,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01341777728988458,"score_gpt":0.2680563756466245,"score_spread":0.25463859835674,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}