{"id":"W3118209894","doi":"10.1021/acschembio.0c00684","title":"Combining Rational Design and Continuous Evolution on Minimalist Proteins That Target the E-box DNA Site","year":2020,"lang":"en","type":"article","venue":"ACS Chemical Biology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":18,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Social Sciences and Humanities Research Council of Canada; Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; University of Toronto","keywords":"Leucine zipper; Zipper; DNA; Computational biology; bZIP domain; Enhancer; Phosphodiester bond; Biology; Transcription factor; Chemistry; Cell biology; Biochemistry; Computer science; RNA; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006251733,0.0005746133,0.0004811287,0.0002903806,0.000184262,0.0005670924,0.0005680009,0.0004080622,0.0005365865],"category_scores_gemma":[0.0004847215,0.0003181199,0.0003448751,0.0002966673,0.0005161709,0.0004315535,0.0005507667,0.0008077283,0.0002666911],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006759301,"about_ca_system_score_gemma":0.0005146523,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003341617,"about_ca_topic_score_gemma":0.0006912055,"domain_scores_codex":[0.9996774,0.00005439631,0.00002371717,0.00007444317,0.0001185175,0.00005151603],"domain_scores_gemma":[0.9998547,0.00004063362,0.00004494349,0.00001718839,0.00002156702,0.00002101926],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002913201,0.0003276094,0.0007879643,0.0003787025,0.00007824178,0.0002501154,0.00009756607,0.02219584,0.9307601,0.01270575,0.0003534199,0.03177327],"study_design_scores_gemma":[0.0001155019,0.001300704,0.0006689951,0.00002737118,0.00005364472,0.0002880491,0.00004709871,0.03743324,0.9405996,0.001890404,0.01753409,0.00004134245],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7516338,0.002515718,0.2382544,0.0004478852,0.0001067897,0.0003910721,0.0002511725,0.0006149103,0.005784171],"genre_scores_gemma":[0.8614091,0.002541289,0.1324794,0.0001319569,0.00002247827,0.0003459196,0.0003563121,0.0001253682,0.002588178],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006759301,"threshold_uncertainty_score":0.00490427,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01341777728988458,"score_gpt":0.2680563756466245,"score_spread":0.25463859835674,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}