{"id":"W3121569222","doi":"10.1101/2021.01.17.427034","title":"Removal of disulfide from acid stress chaperone HdeA does not wholly eliminate structure or function at low pH","year":2021,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Clostridium difficile and Clostridium perfringens research","field":"Medicine","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Division of Chemistry; University of Toronto; National Institutes of Health; National Science Foundation","keywords":"Periplasmic space; Chaperone (clinical); Mutant; Cysteine; Biophysics; Chemistry; Protein folding; Bacteria; Dimer; Biochemistry; Random coil; Protein structure; Protein disulfide-isomerase; Crystallography; Escherichia coli; Cell biology; Disulfide bond; Protein secondary structure; Biology; Genetics; Organic chemistry; Enzyme","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001337768,0.0001869953,0.0001801133,0.00007685123,0.0001087098,0.0001618924,0.0002113825,0.0001596892,0.000840562],"category_scores_gemma":[0.0001405978,0.00005633562,0.000131614,0.0001127577,0.0001633612,0.0001156515,0.0001413644,0.0003333331,0.0002112044],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001866263,"about_ca_system_score_gemma":0.0002138056,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001027296,"about_ca_topic_score_gemma":0.001419227,"domain_scores_codex":[0.9998962,0.00001490676,0.00001181489,0.00001483618,0.00003174444,0.00003057551],"domain_scores_gemma":[0.9998838,0.00001825531,0.00002620714,0.00001975911,0.00002138385,0.00003067639],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008717593,0.00002874195,0.000339943,0.00002200664,0.000005236875,0.00004938501,0.000009883825,0.00007194404,0.998571,0.00005971081,0.00005908634,0.0006958361],"study_design_scores_gemma":[0.0000102016,0.0001432738,0.002697399,0.000004616118,0.000006138003,0.0001572682,0.00002060933,0.0005796036,0.9944535,0.0000238115,0.001899887,0.000003683818],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9976709,0.0003612556,0.001137279,0.00007973719,0.00002060827,0.000006733086,0.0001678911,0.00003884969,0.000516786],"genre_scores_gemma":[0.9969879,0.0001644454,0.0009860239,0.00006000321,0.000003513904,0.000005045407,0.0005202771,0.00001752352,0.001255353],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001027296,"threshold_uncertainty_score":0.002812028,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01602290302402665,"score_gpt":0.24283676444632,"score_spread":0.2268138614222934,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}