{"id":"W3123981312","doi":"10.1002/cpz1.17","title":"Fluorescence Polarization‐Based Measurement of Protein‐Ligand Interaction in Fungal Cell Lysates","year":2021,"lang":"en","type":"article","venue":"Current Protocols","topic":"Antifungal resistance and susceptibility","field":"Medicine","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"National Institute of Allergy and Infectious Diseases; National Institutes of Health; National Science Foundation","keywords":"Lysis; Computational biology; Fluorescence anisotropy; Antifungal; Ligand (biochemistry); Small molecule; Fluorescence; Protein–protein interaction; Biology; Chemistry; Combinatorial chemistry; Biochemistry; Microbiology; Receptor","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004309732,0.0001289851,0.0002739274,0.00008633193,0.00003400423,0.00001980924,0.00007344302,0.00005983804,0.0001003558],"category_scores_gemma":[0.0004075716,0.000115427,0.00009013023,0.0003454002,0.00005060794,0.000119613,0.00002971106,0.0002433306,0.00001228707],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001677198,"about_ca_system_score_gemma":0.0005943119,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000009304749,"about_ca_topic_score_gemma":0.00003385201,"domain_scores_codex":[0.9984346,0.0001089625,0.0004388411,0.0002964929,0.0005329261,0.0001881675],"domain_scores_gemma":[0.998852,0.00002173257,0.0001442173,0.0003419539,0.0005634058,0.00007665367],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004197811,0.001128076,0.3472947,0.001799525,0.00000577361,0.00001007574,0.00007057003,0.000004373379,0.6438926,0.00004510299,0.00005544819,0.005273864],"study_design_scores_gemma":[0.001721911,0.0001832516,0.1346288,0.002767642,0.00001516571,0.000001236984,0.0000769187,0.0002961282,0.8585083,0.00004744226,0.001649925,0.0001032755],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9074565,0.0005096379,0.002076728,0.0003162114,0.0001428068,0.08847342,0.0000170482,0.00005771227,0.0009499501],"genre_scores_gemma":[0.9833904,0.000003018324,0.0006137122,0.00001784975,0.00004058289,0.01579951,0.00002058063,0.00001111993,0.0001032074],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2146157,"threshold_uncertainty_score":0.4706976,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.07075705008452875,"score_gpt":0.3549443752124121,"score_spread":0.2841873251278833,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}