{"id":"W3129007842","doi":"10.3389/fbioe.2021.630551","title":"Challenges Associated With the Formation of Recombinant Protein Inclusion Bodies in Escherichia coli and Strategies to Address Them for Industrial Applications","year":2021,"lang":"en","type":"review","venue":"Frontiers in Bioengineering and Biotechnology","topic":"Protein purification and stability","field":"Biochemistry, Genetics and Molecular Biology","cited_by":297,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph; University of Waterloo; Agriculture and Agri-Food Canada","funders":"Agriculture and Agri-Food Canada","keywords":"Inclusion bodies; Escherichia coli; Recombinant DNA; Inclusion (mineral); Host (biology); Mechanism (biology); Computational biology; Biology; Biochemical engineering; Chemistry; Biotechnology; Biochemistry; Genetics; Gene; Engineering","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009059669,0.0007363152,0.00111263,0.001609238,0.0002791548,0.001249546,0.0007282357,0.001055249,0.001339936],"category_scores_gemma":[0.0007423505,0.0003555191,0.0005067144,0.001649924,0.0005267346,0.001576774,0.0006526874,0.001914129,0.001456949],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006476244,"about_ca_system_score_gemma":0.00106054,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000936248,"about_ca_topic_score_gemma":0.001161855,"domain_scores_codex":[0.9996415,0.00005782055,0.00004266515,0.00005574113,0.0001695335,0.00003270542],"domain_scores_gemma":[0.9996569,0.0001614151,0.0000509337,0.0000134636,0.00009857797,0.00001882812],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"design_other","study_design_gemma":"not_applicable","study_design_scores_codex":[0.00008037265,0.0001017024,0.0002172107,0.03785976,0.0000956531,0.0005262627,0.0001633191,0.001024818,0.03638606,0.01855967,0.01688273,0.8881024],"study_design_scores_gemma":[0.00000637118,0.0001255159,0.0003916525,0.001988528,0.00007804842,0.001352838,0.00007276952,0.0001886393,0.0101516,0.002763424,0.9828551,0.00002554251],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"review","genre_gemma":"review","genre_scores_codex":[0.0004508719,0.9964194,0.001148761,0.0002412444,0.0001650764,0.000008783692,0.00002518828,0.0000158956,0.001524758],"genre_scores_gemma":[0.001537126,0.9961303,0.001103224,0.0001597239,0.00007635854,0.00001389743,0.00005735436,0.000004469623,0.0009174449],"genre_candidate":"review","genre_consensus":"review","teacher_disagreement_score":0.001609238,"threshold_uncertainty_score":0.00479126,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03945958013743522,"score_gpt":0.2700569645048063,"score_spread":0.2305973843673711,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}