{"id":"W3129693673","doi":"10.1089/crispr.2020.0090","title":"Self-Cutting and Integrating CRISPR Plasmids Enable Targeted Genomic Integration of Genetic Payloads for Rapid Cell Engineering","year":2021,"lang":"en","type":"article","venue":"The CRISPR Journal","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Ottawa; National Research Council Canada","funders":"National Research Council Canada","keywords":"CRISPR; Genome editing; Biology; Plasmid; Cas9; Genome engineering; Computational biology; Gene; Genetics; Jurkat cells; Gene targeting; Locus (genetics); T cell","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004842595,0.0006153179,0.0004902988,0.0004478737,0.0002098265,0.0006420078,0.0004813184,0.0005803666,0.001143332],"category_scores_gemma":[0.000379449,0.0002958688,0.0004577573,0.0002822264,0.0004318653,0.0003972972,0.0005105131,0.001108988,0.0008104495],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003520926,"about_ca_system_score_gemma":0.0003099715,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001713515,"about_ca_topic_score_gemma":0.0003521315,"domain_scores_codex":[0.9994824,0.00008368047,0.00004626998,0.0000971378,0.000221385,0.00006920148],"domain_scores_gemma":[0.9996753,0.00007802693,0.0001081508,0.00006492403,0.00003200057,0.00004157905],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000180155,0.00001701092,0.00009734648,0.00006581731,0.000007215128,0.00007974094,0.00001533067,0.0001711747,0.9962745,0.0004734476,0.00006616035,0.002714153],"study_design_scores_gemma":[0.000004272546,0.0001083449,0.0004331043,0.000005635181,0.00001311368,0.0004060942,0.00001047394,0.001139339,0.9927573,0.0001103425,0.005005592,0.000006375018],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5759365,0.006915357,0.4044118,0.0003804484,0.0002137867,0.0006093311,0.001598527,0.002841933,0.007092257],"genre_scores_gemma":[0.8497796,0.004386229,0.135902,0.0001745471,0.00003804517,0.0002547182,0.001791605,0.0002638258,0.007409301],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001143332,"threshold_uncertainty_score":0.00382483,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005579151733347831,"score_gpt":0.2475309501347625,"score_spread":0.2419517984014146,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}