{"id":"W3129693673","doi":"10.1089/crispr.2020.0090","title":"Self-Cutting and Integrating CRISPR Plasmids Enable Targeted Genomic Integration of Genetic Payloads for Rapid Cell Engineering","year":2021,"lang":"en","type":"article","venue":"The CRISPR Journal","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Ottawa; National Research Council Canada","funders":"National Research Council Canada","keywords":"CRISPR; Genome editing; Biology; Plasmid; Cas9; Genome engineering; Computational biology; Gene; Genetics; Jurkat cells; Gene targeting; Locus (genetics); T cell","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004139751,0.0001865945,0.0001941968,0.0000552913,0.0001702461,0.0000757481,0.0001667234,0.0001039894,0.00002001047],"category_scores_gemma":[0.0001922558,0.0001485443,0.0001253528,0.00009110002,0.0000263285,0.00000751556,0.00008961277,0.0002391927,6.302844e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002229603,"about_ca_system_score_gemma":0.00009608168,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000004515492,"about_ca_topic_score_gemma":0.000004072051,"domain_scores_codex":[0.9989347,0.00005286413,0.0003985475,0.0002137438,0.0001096801,0.0002904748],"domain_scores_gemma":[0.9992942,0.00006173107,0.0001397069,0.0002136429,0.0002036318,0.00008713797],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002222069,0.00002255749,0.0002287713,0.00009002383,0.0000651582,0.00000333407,0.0004293587,0.003587629,0.9917393,0.00002888798,0.000361536,0.003421226],"study_design_scores_gemma":[0.0005703669,0.0001783332,0.0005527685,0.00004178841,0.00007844898,0.0002432543,0.0009249357,0.02117356,0.9689693,0.00006341131,0.007024525,0.0001792703],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6932818,0.009886719,0.2960728,0.0001160774,0.0003250059,0.0001596194,0.00001239263,0.00001357682,0.0001320114],"genre_scores_gemma":[0.9372321,0.00125189,0.0608433,0.00005530593,0.000454325,0.00001295291,0.00002099354,0.00003784114,0.0000913089],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2439502,"threshold_uncertainty_score":0.6057462,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005579151733347831,"score_gpt":0.2475309501347625,"score_spread":0.2419517984014146,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}