{"id":"W3133838628","doi":"10.3390/metabo11030147","title":"Developing a Yeast Platform Strain for an Enhanced Taxadiene Biosynthesis by CRISPR/Cas9","year":2021,"lang":"en","type":"article","venue":"Metabolites","topic":"Plant biochemistry and biosynthesis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"Concordia University; University of Calgary","funders":"Genome Alberta; Natural Sciences and Engineering Research Council of Canada; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Genome Canada","keywords":"Yeast; Paclitaxel; Taxus; Biochemistry; Biosynthesis; Saccharomyces cerevisiae; Terpenoid; Geranylgeranyl pyrophosphate; Biology; Chemistry; Gene; Genetics; Botany; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002254118,0.0002478355,0.0002647538,0.00002740672,0.0001420919,0.00006739511,0.0002412122,0.0002213343,0.00004475134],"category_scores_gemma":[0.0004454887,0.0002336469,0.0001428351,0.0001226689,0.00005906031,0.00001207017,0.00007355519,0.00005874564,0.000005511406],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001299267,"about_ca_system_score_gemma":0.0001239796,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000006061889,"about_ca_topic_score_gemma":0.00002765473,"domain_scores_codex":[0.9986039,0.00004203616,0.0002503415,0.0005699652,0.0001128675,0.0004209376],"domain_scores_gemma":[0.9992092,0.0000403397,0.00009584289,0.0003713687,0.0001481537,0.0001350943],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007439467,0.000079383,0.00004603576,0.00003929164,0.00009883278,0.000002227713,0.00001984673,0.000001868091,0.9880869,0.0002092355,0.0007997934,0.01054213],"study_design_scores_gemma":[0.0002756138,0.00005252951,0.0001514987,0.00001735975,0.00005017712,0.00002107088,0.0001855288,0.00003675108,0.9012249,0.00008561232,0.09756923,0.0003297343],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9703873,0.003217154,0.02290584,0.0002231097,0.0001326626,0.0001847405,0.002264472,0.00003688126,0.0006478761],"genre_scores_gemma":[0.9841485,0.0004094385,0.01136075,0.0004612238,0.000320242,0.00006374952,0.002156004,0.00003142093,0.00104869],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.09676944,"threshold_uncertainty_score":0.9527846,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02511022129314977,"score_gpt":0.2699336579247855,"score_spread":0.2448234366316357,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}