{"id":"W3133838628","doi":"10.3390/metabo11030147","title":"Developing a Yeast Platform Strain for an Enhanced Taxadiene Biosynthesis by CRISPR/Cas9","year":2021,"lang":"en","type":"article","venue":"Metabolites","topic":"Plant biochemistry and biosynthesis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"Concordia University; University of Calgary","funders":"Genome Alberta; Natural Sciences and Engineering Research Council of Canada; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Genome Canada","keywords":"Yeast; Paclitaxel; Taxus; Biochemistry; Biosynthesis; Saccharomyces cerevisiae; Terpenoid; Geranylgeranyl pyrophosphate; Biology; Chemistry; Gene; Genetics; Botany; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004912405,0.0008765295,0.0008304983,0.0006091879,0.0002793017,0.0006293014,0.0008291502,0.0005811362,0.001817166],"category_scores_gemma":[0.000404885,0.0004389016,0.0006129202,0.0004962232,0.0002255021,0.0004075986,0.0007972608,0.001321682,0.001599007],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003040597,"about_ca_system_score_gemma":0.0004960702,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001628614,"about_ca_topic_score_gemma":0.002067819,"domain_scores_codex":[0.9995127,0.00004799016,0.0000950886,0.000113986,0.000176901,0.00005328603],"domain_scores_gemma":[0.9997877,0.00004131351,0.00005330014,0.00004027394,0.00003454521,0.00004278222],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005748599,0.00007601587,0.0002889272,0.0001174694,0.00001587776,0.0001447073,0.00004108217,0.000311817,0.9937248,0.0003010738,0.0002062329,0.004714613],"study_design_scores_gemma":[0.00005894341,0.0002808126,0.001991177,0.00003184499,0.00005959421,0.0003722224,0.00007422415,0.005923396,0.9754823,0.0001481894,0.01552995,0.0000473016],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7757006,0.002484376,0.1975621,0.0006611827,0.0005126387,0.001238594,0.008292192,0.005588967,0.007959415],"genre_scores_gemma":[0.7306542,0.002748962,0.2336033,0.0002425928,0.00004416334,0.0009948903,0.01605722,0.001110263,0.01454431],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001817166,"threshold_uncertainty_score":0.006079018,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02511022129314977,"score_gpt":0.2699336579247855,"score_spread":0.2448234366316357,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}