{"id":"W3133952951","doi":"10.17504/protocols.io.4a6gshe","title":"Transposase injection mix protocol v1","year":2019,"lang":"en","type":"preprint","venue":"","topic":"Insect and Arachnid Ecology and Behavior","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"","keywords":"Transposase; Transposable element; Plasmid; Transformation (genetics); Biology; DNA; Aedes aegypti; Genetics; Computational biology; Gene; Genome; Botany","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002618649,0.003119214,0.002957099,0.00476471,0.001961929,0.002170667,0.003467222,0.002286611,0.1042192],"category_scores_gemma":[0.003526293,0.002794156,0.001799841,0.001865011,0.0009572475,0.001101689,0.002567846,0.005598256,0.1352009],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007113964,"about_ca_system_score_gemma":0.001759773,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009971961,"about_ca_topic_score_gemma":0.002437035,"domain_scores_codex":[0.9958852,0.0006190243,0.0006074092,0.001061844,0.001320846,0.0005057259],"domain_scores_gemma":[0.9979618,0.0004426658,0.0001284148,0.0008023394,0.0003878191,0.0002770937],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0009162697,0.0005239102,0.0008816698,0.002299896,0.0001882583,0.000608516,0.0003895158,0.0006745892,0.8008201,0.006445462,0.1000054,0.08624645],"study_design_scores_gemma":[0.0002187579,0.0004285492,0.001828668,0.000284662,0.0001984714,0.001375505,0.00007008364,0.001199038,0.3598708,0.002294358,0.6320623,0.0001689484],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"protocol","genre_scores_codex":[0.02288452,0.006323426,0.6516409,0.001310977,0.003227795,0.02120111,0.1501369,0.07764979,0.06562456],"genre_scores_gemma":[0.0386192,0.007478102,0.3801,0.001972077,0.0004821978,0.03572462,0.3429187,0.03229979,0.1604055],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.1042192,"threshold_uncertainty_score":0.348648,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01363766930019972,"score_gpt":0.2877837881874192,"score_spread":0.2741461188872195,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}