{"id":"W3134079052","doi":"10.1261/rna.078493.120","title":"RNA Peach and Mango: orthogonal two-color fluorogenic aptamers distinguish nearly identical ligands","year":2021,"lang":"en","type":"article","venue":"RNA","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":25,"is_retracted":false,"has_abstract":true,"ca_institutions":"Simon Fraser University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Aptamer; Biotin; RNA; Biology; SELEX Aptamer Technique; Fluorophore; Biotinylation; Systematic evolution of ligands by exponential enrichment; Biochemistry; Oligonucleotide; In vitro; Biophysics; Fluorescence; Computational biology; Genetics; DNA; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004602403,0.0004008393,0.000307004,0.0002353112,0.0001318341,0.0004311004,0.0003074791,0.0004856215,0.0008028586],"category_scores_gemma":[0.0004736407,0.0002796556,0.000169435,0.0001462267,0.0004246819,0.0003010236,0.0003672727,0.0004788184,0.0004084734],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003522263,"about_ca_system_score_gemma":0.00020605,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002798658,"about_ca_topic_score_gemma":0.0009577269,"domain_scores_codex":[0.9994169,0.0001230605,0.00001864451,0.000174012,0.000155327,0.0001120407],"domain_scores_gemma":[0.9996742,0.0001048971,0.00009295939,0.00003424589,0.00003685494,0.00005689413],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003106957,0.000004147665,0.00006231352,0.00001198107,0.000001289237,0.00001080111,0.000009548864,0.00003585289,0.9990958,0.0001141976,0.00001434546,0.0006086582],"study_design_scores_gemma":[0.000006592756,0.0000822822,0.0009738948,0.000003441269,0.000003361882,0.0001409704,0.00001436228,0.0009177064,0.9966133,0.00008348619,0.001154336,0.000006382738],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9310226,0.001140034,0.06324426,0.0001238404,0.00005780343,0.0001112071,0.0002022346,0.0003240789,0.003773904],"genre_scores_gemma":[0.9300261,0.0005040697,0.0617453,0.0002240624,0.00002707281,0.0001581756,0.0003150222,0.0001056496,0.006894618],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008028586,"threshold_uncertainty_score":0.002685785,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007806679673179501,"score_gpt":0.273810446920059,"score_spread":0.2660037672468795,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}