{"id":"W3134079052","doi":"10.1261/rna.078493.120","title":"RNA Peach and Mango: orthogonal two-color fluorogenic aptamers distinguish nearly identical ligands","year":2021,"lang":"en","type":"article","venue":"RNA","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":25,"is_retracted":false,"has_abstract":true,"ca_institutions":"Simon Fraser University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Aptamer; Biotin; RNA; Biology; SELEX Aptamer Technique; Fluorophore; Biotinylation; Systematic evolution of ligands by exponential enrichment; Biochemistry; Oligonucleotide; In vitro; Biophysics; Fluorescence; Computational biology; Genetics; DNA; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001520502,0.0001667746,0.0001890129,0.00002993587,0.0001200026,0.00006608768,0.00009749365,0.0001282463,0.000007136118],"category_scores_gemma":[0.0001978266,0.0001545203,0.0001428568,0.0001327657,0.0001470348,0.000004260548,0.0001656226,0.0001225805,0.000004265253],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001308148,"about_ca_system_score_gemma":0.00005671335,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000009318425,"about_ca_topic_score_gemma":0.00005167801,"domain_scores_codex":[0.9988777,0.00003750021,0.0002195711,0.0004514733,0.0001514669,0.0002623041],"domain_scores_gemma":[0.9993725,0.00001463104,0.00007111138,0.0002894505,0.0001313652,0.0001209374],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003235227,0.00004577018,0.001609577,0.00001074179,0.00009132238,0.00006293556,0.00001491733,6.0514e-7,0.9855276,0.0001683581,0.0006160358,0.01181976],"study_design_scores_gemma":[0.0002819515,0.0001082312,0.002032615,0.00001952535,0.00009221875,0.0001194466,0.00006485644,0.0001176984,0.9893724,0.0001868215,0.007357809,0.0002463673],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9968618,0.0008106713,0.000687187,0.0002720263,0.00007181428,0.00005792936,0.00001724785,0.00004478312,0.001176559],"genre_scores_gemma":[0.9926826,0.0002757031,0.005008451,0.0003501242,0.0003526525,0.000004598518,0.0001404637,0.00002110833,0.001164285],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01157339,"threshold_uncertainty_score":0.6301156,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007806679673179501,"score_gpt":0.273810446920059,"score_spread":0.2660037672468795,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}