{"id":"W3134318926","doi":"10.1186/s13073-021-00859-1","title":"SARS-CoV-2 vaccine ChAdOx1 nCoV-19 infection of human cell lines reveals low levels of viral backbone gene transcription alongside very high levels of SARS-CoV-2 S glycoprotein gene transcription","year":2021,"lang":"en","type":"article","venue":"Genome Medicine","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":61,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Biotechnology and Biological Sciences Research Council; Engineering and Physical Sciences Research Council; U.S. Food and Drug Administration; Hamilton Health Sciences Foundation","keywords":"Biology; Virology; Gene; Transcriptome; Cell culture; Viral replication; Proteome; Glycoprotein; Viral vector; Transcription (linguistics); Polyadenylation; Gene expression; Genetics; Virus; Recombinant DNA","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001194779,0.0002344396,0.0001852286,0.0001913749,0.0001438183,0.000321005,0.00007672506,0.000241901,0.001878205],"category_scores_gemma":[0.0001583971,0.0001198279,0.0002336252,0.0002221216,0.0002025307,0.000158225,0.0001297298,0.0002838344,0.0007817764],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002191696,"about_ca_system_score_gemma":0.0001620681,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00110246,"about_ca_topic_score_gemma":0.001176891,"domain_scores_codex":[0.9998478,0.00001321488,0.00001431638,0.00004614832,0.00005219271,0.00002635548],"domain_scores_gemma":[0.9999113,0.00002245456,0.00001950774,0.0000139737,0.00002182009,0.00001089404],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003894295,0.000005070221,0.0004484532,0.00001904451,0.000002798627,0.00002517912,0.00001589732,0.00001654344,0.9990707,0.00001663506,0.00003051544,0.0003102987],"study_design_scores_gemma":[0.000006760032,0.0003379715,0.04120568,0.00001065104,0.00002036987,0.0005799686,0.0001496138,0.0004520579,0.9539146,0.00003888116,0.003275524,0.000007940084],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.987069,0.001581961,0.004210738,0.0001429797,0.00003130041,0.00004126158,0.004331477,0.0000925659,0.002498661],"genre_scores_gemma":[0.9793636,0.00175273,0.004670462,0.000168689,0.00001740673,0.0000887835,0.009453525,0.00004537122,0.004439389],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001878205,"threshold_uncertainty_score":0.006283224,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05012344341403854,"score_gpt":0.3194673449097172,"score_spread":0.2693439014956787,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}