{"id":"W3134581659","doi":"10.17504/protocols.io.4v7gw9n","title":"How to Prepare a Single Cell Suspension from Mouse Spleen v1","year":2019,"lang":"en","type":"preprint","venue":"","topic":"Immunotoxicology and immune responses","field":"Immunology and Microbiology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Stemcell Technologies","funders":"","keywords":"Spleen; Immune system; Cell; Haematopoiesis; Immunology; Hematopoietic cell; Suspension culture; Suspension (topology); Blood cell; Lymphatic system; Biology; Antigen; Cell biology; Cell culture; Stem cell; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009880974,0.00108899,0.000743711,0.001737489,0.0007402686,0.0007899307,0.0009329497,0.0009561841,0.01428873],"category_scores_gemma":[0.0006715738,0.0009906518,0.0008242591,0.0007141614,0.000420484,0.0005918792,0.000625308,0.002415906,0.02074682],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003365056,"about_ca_system_score_gemma":0.0005126685,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005430495,"about_ca_topic_score_gemma":0.001488079,"domain_scores_codex":[0.9991867,0.00007109857,0.00009946401,0.0002486898,0.0002758758,0.0001182202],"domain_scores_gemma":[0.9994752,0.0001194355,0.00005182731,0.0001158903,0.0001358046,0.0001018226],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.00008536627,0.00008024659,0.000204191,0.0002018978,0.00001473628,0.000123089,0.00006113281,0.0001686007,0.9865063,0.0007422323,0.002351714,0.009460479],"study_design_scores_gemma":[0.00006720863,0.0003601787,0.002808535,0.0001121254,0.00006193291,0.0004699507,0.0000464486,0.00137421,0.8874199,0.0008470407,0.10639,0.00004262181],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1679736,0.01233693,0.6901736,0.002298023,0.003813327,0.01036404,0.03631935,0.01495813,0.06176302],"genre_scores_gemma":[0.1768916,0.01233121,0.6317655,0.001703289,0.0008808257,0.01611229,0.0748909,0.005190802,0.0802336],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01428873,"threshold_uncertainty_score":0.04780054,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01884308015220763,"score_gpt":0.2346964532526116,"score_spread":0.215853373100404,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}