{"id":"W3134932213","doi":"10.1002/advs.202004632","title":"Construction of an Alternative NAD <sup>+</sup> De Novo Biosynthesis Pathway","year":2021,"lang":"en","type":"article","venue":"Advanced Science","topic":"Enzyme Catalysis and Immobilization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":31,"is_retracted":false,"has_abstract":true,"ca_institutions":"Wilfrid Laurier University","funders":"National Natural Science Foundation of China","keywords":"NAD+ kinase; Nicotinamide adenine dinucleotide; Cofactor; Biosynthesis; Biochemistry; Nicotinamide phosphoribosyltransferase; De novo synthesis; Chemistry; Amino acid; Metabolic pathway; Bioconversion; Enzyme; Biology; Stereochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001841271,0.00006622499,0.00008011821,0.00003885637,0.00008736584,0.00001581022,0.0001678203,0.00003554654,0.00001415207],"category_scores_gemma":[0.0003599446,0.00006437391,0.00003400448,0.0003312561,0.0003302016,0.00002713988,0.00005774046,0.0000286486,0.000001434521],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002069618,"about_ca_system_score_gemma":0.0002275054,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000005132376,"about_ca_topic_score_gemma":0.00001038262,"domain_scores_codex":[0.9992337,0.0000318785,0.0001247463,0.0003204876,0.0001388004,0.0001503747],"domain_scores_gemma":[0.9993019,0.000009722393,0.00007112737,0.0002874849,0.0002699234,0.00005983376],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00000871859,0.00002959114,0.0003239902,0.000004014365,0.000004488668,0.000001572027,0.00007148873,0.003964771,0.9801993,0.0004816765,0.000001514552,0.01490884],"study_design_scores_gemma":[0.0001359834,0.00007805436,0.0003186436,0.00001028313,0.000004466101,0.00002732005,0.0004529541,0.00180511,0.9958876,0.0002006759,0.001000585,0.00007826841],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9892534,0.000163277,0.009916142,0.00002506404,0.00005394871,0.00004152517,0.00001539076,0.000005182098,0.0005260576],"genre_scores_gemma":[0.9840257,0.00009578287,0.01563921,0.00007493317,0.00003459454,0.000004815099,0.00003500754,0.00000530734,0.00008460921],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01568833,"threshold_uncertainty_score":0.2625092,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007428395648995754,"score_gpt":0.2537636786352996,"score_spread":0.2463352829863038,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}