{"id":"W3135320303","doi":"10.1038/s41598-021-84618-7","title":"Identification of an alpha-1 antitrypsin variant with enhanced specificity for factor XIa by phage display, bacterial expression, and combinatorial mutagenesis","year":2021,"lang":"en","type":"article","venue":"Scientific Reports","topic":"Coagulation, Bradykinin, Polyphosphates, and Angioedema","field":"Medicine","cited_by":11,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University; Canadian Blood Services","funders":"Health Canada; Heart and Stroke Foundation of Canada; Australian Government; Government of Canada; International Society on Thrombosis and Haemostasis; Canadian Blood Services; McMaster University","keywords":"Serpin; Proteases; Phage display; Bacteriophage; Molecular biology; Biology; Directed evolution; Mutagenesis; Protease; Thrombin; Serine protease; Biochemistry; Escherichia coli; Gene; Mutant; Peptide; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001924191,0.0003303769,0.000302251,0.0001845548,0.0001126682,0.000356594,0.0002165112,0.0002470156,0.0004062752],"category_scores_gemma":[0.0002270484,0.0001398063,0.000327147,0.0003062697,0.0001534775,0.00009586846,0.0002246262,0.0004862881,0.0002233525],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003135674,"about_ca_system_score_gemma":0.0001483398,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005898488,"about_ca_topic_score_gemma":0.0009378966,"domain_scores_codex":[0.9997739,0.00004580839,0.00002129128,0.00004245656,0.00006496079,0.00005152857],"domain_scores_gemma":[0.9999132,0.00002029796,0.00002434079,0.00001136894,0.00001246353,0.00001829771],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007687497,0.00003476486,0.0001514046,0.00001383738,0.000006095463,0.00004604378,0.00001138609,0.00021807,0.9980901,0.00007104168,0.00002345534,0.001257037],"study_design_scores_gemma":[0.00001493146,0.0003762899,0.001593914,0.000002797037,0.00002311589,0.0003997133,0.00001349771,0.002363704,0.9940377,0.00001759591,0.001149295,0.000007333079],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9941376,0.0002730213,0.004722279,0.00003913814,0.00000753964,0.00004161774,0.0001629864,0.00005569941,0.0005600583],"genre_scores_gemma":[0.9901362,0.0002568972,0.008059163,0.00003195374,0.000004222008,0.00002849001,0.0004823536,0.00003413162,0.0009666034],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005898488,"threshold_uncertainty_score":0.002275109,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01109568187072326,"score_gpt":0.259489611358126,"score_spread":0.2483939294874028,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}