{"id":"W3135394976","doi":"10.17504/protocols.io.vcme2u6","title":"In vitro synthesized mRNA transfection to Perkinsus marinus v1","year":2018,"lang":"en","type":"preprint","venue":"","topic":"Animal Genetics and Reproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"","keywords":"In vitro; Transfection; Messenger RNA; Chemistry; Cell biology; Biology; Molecular biology; Biochemistry; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007028697,0.001110039,0.001080961,0.0007288036,0.0005933456,0.0007132364,0.001007559,0.0007535718,0.005326844],"category_scores_gemma":[0.0003276161,0.000677943,0.0007533169,0.0006396875,0.0005518466,0.0003276643,0.0007237493,0.00213666,0.003081662],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009360866,"about_ca_system_score_gemma":0.0005229281,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001767775,"about_ca_topic_score_gemma":0.003364217,"domain_scores_codex":[0.9993576,0.00008103099,0.00004456141,0.0002464899,0.0001483104,0.0001219721],"domain_scores_gemma":[0.9997897,0.00005558466,0.00003312613,0.00003896235,0.00003984032,0.00004288885],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003665739,0.00003328936,0.00003888166,0.0001466055,0.000005502084,0.00005259961,0.0000720469,0.00009517177,0.9974165,0.0003892186,0.0002750406,0.001438599],"study_design_scores_gemma":[0.0000286196,0.0002917349,0.0008254863,0.00003096926,0.0000348665,0.0001460624,0.00005443725,0.001191563,0.9569183,0.0001619836,0.04029929,0.00001668393],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7585682,0.01273637,0.1715048,0.0008043784,0.002672472,0.00223108,0.01147188,0.004368587,0.03564231],"genre_scores_gemma":[0.7404532,0.006435503,0.1165001,0.0007344907,0.0003945399,0.001635223,0.02435313,0.002565404,0.1069284],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.005326844,"threshold_uncertainty_score":0.01782006,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01096452570462891,"score_gpt":0.2530636484953532,"score_spread":0.2420991227907243,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}