{"id":"W3135394976","doi":"10.17504/protocols.io.vcme2u6","title":"In vitro synthesized mRNA transfection to Perkinsus marinus v1","year":2018,"lang":"en","type":"preprint","venue":"","topic":"Animal Genetics and Reproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"","keywords":"In vitro; Transfection; Messenger RNA; Chemistry; Cell biology; Biology; Molecular biology; Biochemistry; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0002438848,0.0002651842,0.0002446023,0.0001176818,0.00004603929,0.00003641458,0.000215402,0.0004939379,0.00008693188],"category_scores_gemma":[0.00005363803,0.0002482536,0.000157027,0.00008608714,0.00004880937,0.000001184345,0.0003258748,0.0002106444,0.00005744528],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000410721,"about_ca_system_score_gemma":0.00008940987,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002962267,"about_ca_topic_score_gemma":0.0001498432,"domain_scores_codex":[0.9983177,0.00006624435,0.0002805757,0.0009360439,0.0001349497,0.0002644805],"domain_scores_gemma":[0.999155,0.000003923091,0.00005488524,0.0005882476,0.0001044894,0.00009345536],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006168682,0.0000866293,0.00008384058,0.00004369826,0.00004551252,0.00000361444,0.0000549541,0.0001763767,0.9924893,0.000007764057,0.001806443,0.00458499],"study_design_scores_gemma":[0.0002803851,0.0003051149,0.00205136,0.00002226642,0.00002668913,0.00001837605,0.00002673322,0.0000891806,0.9307869,0.0001593256,0.06585911,0.0003745303],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.986747,0.0003323334,0.003941171,0.0006694031,0.001353755,0.0006191919,0.00002065263,0.00002405635,0.006292408],"genre_scores_gemma":[0.9902721,0.0003661446,0.002452427,0.0002561531,0.001515122,0.00009152324,0.00009467403,0.00004394911,0.004907903],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.06405266,"threshold_uncertainty_score":0.999997,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01096452570462891,"score_gpt":0.2530636484953532,"score_spread":0.2420991227907243,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}