{"id":"W3139236263","doi":"10.1186/s12896-021-00714-6","title":"Expansion of targetable sites for the ribonucleoprotein-based CRISPR/Cas9 system in the silkworm Bombyx mori","year":2021,"lang":"en","type":"article","venue":"BMC Biotechnology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":11,"is_retracted":false,"has_abstract":true,"ca_institutions":"Ministry of Agriculture","funders":"Fundamental Research Funds for the Central Universities; National Natural Science Foundation of China","keywords":"CRISPR; Biology; Cas9; Subgenomic mRNA; Genome editing; Genetics; Ribonucleoprotein; Mutant; Gene; Mutagenesis; Genome engineering; Computational biology; Transcription activator-like effector nuclease; Bombyx; Gene targeting; Bombyx mori; RNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003809433,0.0005193267,0.0003924198,0.0003239874,0.0002537738,0.0003389683,0.0006152021,0.0006359242,0.0008810977],"category_scores_gemma":[0.0002153905,0.0003047934,0.0004604507,0.0001928234,0.0002213465,0.0002091852,0.0004656549,0.0008540306,0.0007443598],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005874534,"about_ca_system_score_gemma":0.0003860211,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009709789,"about_ca_topic_score_gemma":0.001534606,"domain_scores_codex":[0.9994991,0.00005468159,0.00007800067,0.0001158025,0.0001961924,0.00005611971],"domain_scores_gemma":[0.9998065,0.00002522853,0.00007115862,0.00002794388,0.00002411878,0.00004521495],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002788966,0.00002801462,0.0001591177,0.00005396701,0.000005117099,0.00004850872,0.00001503048,0.0001417016,0.998173,0.00007810874,0.00002750386,0.001242039],"study_design_scores_gemma":[0.00002184609,0.0002170282,0.002381593,0.00001586042,0.00004140681,0.0002572537,0.00002149633,0.002589958,0.9901118,0.00003772683,0.004289973,0.00001416659],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.953006,0.001547711,0.04107255,0.0001419796,0.00007893812,0.000491418,0.001111056,0.0005046764,0.002045649],"genre_scores_gemma":[0.9188461,0.001047615,0.07377617,0.0001021648,0.00001750493,0.0003016109,0.002986493,0.0001426104,0.002779786],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009709789,"threshold_uncertainty_score":0.004262269,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01241743188482666,"score_gpt":0.2752854905066799,"score_spread":0.2628680586218533,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}