{"id":"W3139236263","doi":"10.1186/s12896-021-00714-6","title":"Expansion of targetable sites for the ribonucleoprotein-based CRISPR/Cas9 system in the silkworm Bombyx mori","year":2021,"lang":"en","type":"article","venue":"BMC Biotechnology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":11,"is_retracted":false,"has_abstract":true,"ca_institutions":"Ministry of Agriculture","funders":"Fundamental Research Funds for the Central Universities; National Natural Science Foundation of China","keywords":"CRISPR; Biology; Cas9; Subgenomic mRNA; Genome editing; Genetics; Ribonucleoprotein; Mutant; Gene; Mutagenesis; Genome engineering; Computational biology; Transcription activator-like effector nuclease; Bombyx; Gene targeting; Bombyx mori; RNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002942675,0.0001335546,0.0001761058,0.00005256997,0.00007210141,0.00001165225,0.0003266207,0.0003367505,0.000005159646],"category_scores_gemma":[0.0001815786,0.00008956457,0.0001095376,0.0002247759,0.00008942216,0.000001474804,0.00008687707,0.0001310559,0.000001884791],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001069769,"about_ca_system_score_gemma":0.00008517245,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000331042,"about_ca_topic_score_gemma":0.0002038307,"domain_scores_codex":[0.9991096,0.00004032435,0.0002299821,0.0002782614,0.00009045065,0.0002514328],"domain_scores_gemma":[0.9991013,0.00007898527,0.00006384711,0.000670733,0.00006898235,0.00001617662],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004293144,0.00004051325,0.0007485814,0.0001834746,0.00002043232,0.000003704467,0.00002754624,0.002334932,0.9930741,0.001940244,0.0003943302,0.001189278],"study_design_scores_gemma":[0.0004919596,0.0001424844,0.0007708422,0.00002542951,0.00002379856,0.00002066924,0.000826441,0.006529623,0.9773355,0.00005192022,0.01366941,0.0001118969],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5424225,0.01198239,0.4427873,0.001577689,0.0002388632,0.0007943723,0.00004229724,0.00005019876,0.0001043888],"genre_scores_gemma":[0.9892814,0.00009141157,0.01018253,0.000110449,0.00007620703,0.0001247965,0.00004274029,0.00001819018,0.00007229773],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.4468589,"threshold_uncertainty_score":0.3652337,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01241743188482666,"score_gpt":0.2752854905066799,"score_spread":0.2628680586218533,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}