{"id":"W3143312462","doi":"10.21203/rs.3.pex-1438/v1","title":"cpn60 metagenomic amplicon library preparation for the Illumina Miseq platform","year":2021,"lang":"en","type":"preprint","venue":"Research Square","topic":"Microbial infections and disease research","field":"Immunology and Microbiology","cited_by":11,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Saskatchewan","funders":"","keywords":"Biology; Amplicon; DNA sequencing; Computational biology; Primer (cosmetics); Genetics; Polymerase chain reaction; Gene; Chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow","sts","insufficient_payload"],"consensus_categories":[],"category_scores_codex":[0.001572055,0.0003315342,0.0004670258,0.0004285419,0.00133454,0.0006157015,0.0008327544,0.0007993053,0.005305346],"category_scores_gemma":[0.0004978959,0.0002483827,0.0005721549,0.000400477,0.0005634137,0.0002514498,0.001957185,0.002002065,0.0005693421],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000202808,"about_ca_system_score_gemma":0.001641588,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005398003,"about_ca_topic_score_gemma":0.0001389362,"domain_scores_codex":[0.9966702,0.0007580763,0.000465724,0.0009048678,0.0001792161,0.001021953],"domain_scores_gemma":[0.9961037,0.00173341,0.0001228472,0.00136023,0.0005816748,0.00009811041],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.003169957,0.001635776,0.0007949527,0.004647062,0.003856694,0.00005468554,0.003480394,0.0008660847,0.5153651,0.009484274,0.4178274,0.03881765],"study_design_scores_gemma":[0.002199223,0.0007442576,0.002604624,0.0007484331,0.0002685007,0.00007486613,0.004337579,0.0004819286,0.193869,0.003018172,0.7907639,0.0008895188],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8566002,0.1083463,0.002707746,0.003864815,0.002645664,0.01301256,0.003212022,0.0004465745,0.009164132],"genre_scores_gemma":[0.9663063,0.004720632,0.0001488081,0.00008639924,0.0004084392,0.00254471,0.005790224,0.00009690349,0.01989756],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.3729365,"threshold_uncertainty_score":0.9999968,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.09055143557178429,"score_gpt":0.3970713620131229,"score_spread":0.3065199264413386,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}