{"id":"W3143312462","doi":"10.21203/rs.3.pex-1438/v1","title":"cpn60 metagenomic amplicon library preparation for the Illumina Miseq platform","year":2021,"lang":"en","type":"preprint","venue":"Research Square","topic":"Microbial infections and disease research","field":"Immunology and Microbiology","cited_by":11,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Saskatchewan","funders":"","keywords":"Biology; Amplicon; DNA sequencing; Computational biology; Primer (cosmetics); Genetics; Polymerase chain reaction; Gene; Chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001537923,0.002470776,0.002363621,0.002359821,0.00201911,0.001723768,0.001673053,0.001348149,0.02259118],"category_scores_gemma":[0.003176656,0.001820357,0.002234531,0.002335321,0.0005715229,0.0008568325,0.001894553,0.003868116,0.02799246],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008397388,"about_ca_system_score_gemma":0.002505399,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002526597,"about_ca_topic_score_gemma":0.006254721,"domain_scores_codex":[0.9978693,0.0001724839,0.0001523726,0.0008137088,0.0006411128,0.0003509552],"domain_scores_gemma":[0.9991791,0.0001609438,0.00005224587,0.000205069,0.000263816,0.0001387279],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000765498,0.0001516625,0.001487438,0.001218006,0.0002062877,0.0002325012,0.0003106002,0.001518932,0.9117643,0.003435959,0.03294078,0.0459681],"study_design_scores_gemma":[0.0001493468,0.0005388478,0.008312067,0.0002402801,0.0002518623,0.0004913042,0.0001123443,0.009516723,0.6572222,0.005454536,0.3174935,0.0002170847],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.07217932,0.003148013,0.5690974,0.0009189562,0.002504911,0.005070952,0.2729678,0.03478312,0.03932949],"genre_scores_gemma":[0.0615651,0.001736234,0.5279168,0.001777424,0.0004683437,0.007660784,0.3571463,0.01088208,0.03084693],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.02259118,"threshold_uncertainty_score":0.07557499,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.09055143557178429,"score_gpt":0.3970713620131229,"score_spread":0.3065199264413386,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}