{"id":"W3153779616","doi":"10.1007/s12010-021-03573-1","title":"Optimisation of Cytochrome P450 BM3 Assisted by Consensus-Guided Evolution","year":2021,"lang":"en","type":"article","venue":"Applied Biochemistry and Biotechnology","topic":"Pharmacogenetics and Drug Metabolism","field":"Pharmacology, Toxicology and Pharmaceutics","cited_by":8,"is_retracted":false,"has_abstract":false,"ca_institutions":"Université Laval","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Cofactor; Bacillus megaterium; Mutant; Chemistry; Thermostability; Enzyme; Protein engineering; Directed evolution; Biochemistry; Reductase; Oxidase test; Oxidoreductase; Stereochemistry; Biology; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006445828,0.0009261042,0.001078399,0.0003489348,0.0004299632,0.0007111378,0.000762586,0.000780819,0.002708195],"category_scores_gemma":[0.001550882,0.0002604957,0.0007185513,0.0004966067,0.000270232,0.0002953215,0.0006247247,0.001164183,0.0006703457],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006433403,"about_ca_system_score_gemma":0.001036724,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002580721,"about_ca_topic_score_gemma":0.003570641,"domain_scores_codex":[0.9996542,0.0001155744,0.00001625497,0.00007714215,0.00007799522,0.00005901886],"domain_scores_gemma":[0.9997833,0.0001025307,0.00002066064,0.0000174244,0.00005404903,0.00002215527],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"simulation_or_modeling","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.002215676,0.0007575296,0.004137005,0.0007929203,0.0003851172,0.0009943927,0.0002785719,0.5214834,0.2110187,0.009179311,0.003957706,0.2447997],"study_design_scores_gemma":[0.0002773709,0.0007169728,0.0008345578,0.0000296551,0.0001448632,0.0002262685,0.00009747096,0.9406576,0.04851419,0.00196169,0.006498,0.00004134531],"study_design_candidate":"bench_or_experimental","study_design_consensus":null,"genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7266747,0.00233424,0.2572158,0.0005194995,0.0002153955,0.0002673805,0.0004610597,0.002164581,0.0101475],"genre_scores_gemma":[0.8926209,0.0005737683,0.1031118,0.0001669373,0.00002053638,0.0001754997,0.0007391509,0.000260332,0.002331083],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002708195,"threshold_uncertainty_score":0.009059846,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04895245608808637,"score_gpt":0.3611584704064935,"score_spread":0.3122060143184072,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}