{"id":"W3153779616","doi":"10.1007/s12010-021-03573-1","title":"Optimisation of Cytochrome P450 BM3 Assisted by Consensus-Guided Evolution","year":2021,"lang":"en","type":"article","venue":"Applied Biochemistry and Biotechnology","topic":"Pharmacogenetics and Drug Metabolism","field":"Pharmacology, Toxicology and Pharmaceutics","cited_by":8,"is_retracted":false,"has_abstract":false,"ca_institutions":"Université Laval","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Cofactor; Bacillus megaterium; Mutant; Chemistry; Thermostability; Enzyme; Protein engineering; Directed evolution; Biochemistry; Reductase; Oxidase test; Oxidoreductase; Stereochemistry; Biology; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002736675,0.0002302612,0.0003407818,0.00006730441,0.0001437444,0.000009162071,0.0001810725,0.001105163,0.0002699729],"category_scores_gemma":[0.00007752058,0.0002415079,0.00006410064,0.0003361288,0.0006240748,0.00001294934,0.0001646776,0.0005323873,0.00001281972],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004311284,"about_ca_system_score_gemma":0.0001306586,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000005112806,"about_ca_topic_score_gemma":8.054102e-7,"domain_scores_codex":[0.9985742,0.00007335564,0.0004210301,0.0004769909,0.0001146827,0.0003397604],"domain_scores_gemma":[0.999153,0.0001052985,0.000215288,0.0002770807,0.0001154317,0.0001339118],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009093161,0.0002242488,0.0000931924,0.00008478958,0.0001354159,0.00001383608,0.00002624092,0.00001234774,0.9790646,0.002951916,0.004864865,0.01243762],"study_design_scores_gemma":[0.001509939,0.00002331683,0.0002741935,0.00000713338,0.0001483795,0.0001619917,0.000144342,0.0002010401,0.9537274,0.000865284,0.04269937,0.0002375741],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9891587,0.003850297,0.0002343708,0.002193259,0.0001834266,0.0001899899,0.0001901986,0.0001160064,0.003883735],"genre_scores_gemma":[0.9965637,0.0009307633,0.001188532,0.0004829798,0.00007872791,0.00003076565,0.0001639967,0.00001578362,0.0005447345],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03783451,"threshold_uncertainty_score":0.9848407,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04895245608808637,"score_gpt":0.3611584704064935,"score_spread":0.3122060143184072,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}