{"id":"W3156657399","doi":"10.1101/2021.04.11.439384","title":"CRISPR Knock-in Designer: automatic oligonucleotide design software to introduce point mutations using CRISPR/Cas9","year":2021,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Ottawa; Sunnybrook Health Science Centre; Children's Hospital of Eastern Ontario","funders":"","keywords":"CRISPR; Genome editing; Cas9; Computational biology; Biology; Point mutation; Genetics; Oligonucleotide; Gene; Context (archaeology); Computer science; Mutation","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001299582,0.001063913,0.0008597624,0.000739321,0.000348205,0.0006719429,0.001108938,0.0006234347,0.009891589],"category_scores_gemma":[0.001106822,0.0009788148,0.0009151258,0.0003064747,0.0003677364,0.0005137899,0.0006557844,0.001197707,0.006232036],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003473135,"about_ca_system_score_gemma":0.0005488587,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000351753,"about_ca_topic_score_gemma":0.0005684396,"domain_scores_codex":[0.9993199,0.00008955503,0.00009844874,0.0001950083,0.000246766,0.000050446],"domain_scores_gemma":[0.9993174,0.0002698702,0.0001479857,0.00009608617,0.00009855784,0.00007019109],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0007044927,0.0001456523,0.001555484,0.001267215,0.0002133185,0.0005084943,0.0002269177,0.008523254,0.8540358,0.005109258,0.02364146,0.1040687],"study_design_scores_gemma":[0.0002549457,0.0004427584,0.001463954,0.0001117094,0.0001324284,0.0009118671,0.0000330864,0.04330663,0.8065874,0.002087473,0.1444917,0.0001760915],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"software","genre_scores_codex":[0.01791318,0.0003516098,0.884187,0.00007767017,0.0001337807,0.0003092108,0.003603569,0.09025666,0.003167314],"genre_scores_gemma":[0.0994146,0.0006920809,0.8556078,0.0003505528,0.00003635427,0.001549184,0.0100344,0.01459842,0.01771661],"genre_candidate":"software","genre_consensus":null,"teacher_disagreement_score":0.009891589,"threshold_uncertainty_score":0.03309071,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01526819801682057,"score_gpt":0.2701409469980115,"score_spread":0.2548727489811909,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}