{"id":"W3157420610","doi":"10.1038/s41596-021-00515-3","title":"Microtiter plate assays to assess antibiofilm activity against bacteria","year":2021,"lang":"en","type":"article","venue":"Nature Protocols","topic":"Bacterial biofilms and quorum sensing","field":"Biochemistry, Genetics and Molecular Biology","cited_by":267,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of British Columbia","funders":"Canadian Institutes of Health Research; Government of Canada","keywords":"Biofilm; Microtiter plate; Crystal violet; Microbiology; Plate reader; Tetrazolium chloride; Formazan; Bacteria; Resazurin; Chemistry; Biology; Biochemistry; Chromatography; Medicine","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002543551,0.007003228,0.002978363,0.004001903,0.001360942,0.001040809,0.004265007,0.002118947,0.00938603],"category_scores_gemma":[0.001985707,0.002371118,0.002086152,0.004017627,0.001025109,0.0009441372,0.001835587,0.005413936,0.007896533],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00065816,"about_ca_system_score_gemma":0.001448096,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00155092,"about_ca_topic_score_gemma":0.005172806,"domain_scores_codex":[0.9900379,0.002118784,0.001221258,0.0009346366,0.00500561,0.0006817436],"domain_scores_gemma":[0.9966596,0.001178655,0.0004198757,0.0006003745,0.0009689165,0.0001726195],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001156563,0.0003376999,0.0002483575,0.0008345719,0.00008314137,0.00007377939,0.0001866443,0.0001713826,0.9871947,0.000908773,0.002099917,0.007745438],"study_design_scores_gemma":[0.0000642154,0.0008277223,0.002355615,0.0001002959,0.0001429153,0.000226159,0.00007735174,0.001408318,0.9843974,0.0004928313,0.009838378,0.00006888184],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1642606,0.01353658,0.7301311,0.001436691,0.002357775,0.007997593,0.01625215,0.01274168,0.05128583],"genre_scores_gemma":[0.1211578,0.01381516,0.7417645,0.0009343613,0.0003840463,0.01884873,0.02427972,0.001180513,0.07763522],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.00938603,"threshold_uncertainty_score":0.03139943,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02103228576030133,"score_gpt":0.3153547028852563,"score_spread":0.294322417124955,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}