{"id":"W3160758484","doi":"10.1101/2021.05.12.443385","title":"Functional expression of opioid receptors and other human GPCRs in yeast engineered to produce human sterols","year":2021,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Concordia University","funders":"","keywords":"G protein-coupled receptor; Sterol; DAMGO; Receptor; Biology; Yeast; Agonist; Opioid; Saccharomyces cerevisiae; Chemistry; Biochemistry; Opioid receptor; Cholesterol","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003713913,0.0004616785,0.0003092501,0.0002311297,0.0001341643,0.0004599033,0.0003362672,0.0002511456,0.0008350979],"category_scores_gemma":[0.0002531165,0.0001803161,0.0002764941,0.000339253,0.0002131776,0.0001320382,0.0002994604,0.0004603349,0.0005345698],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004128593,"about_ca_system_score_gemma":0.0002340273,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001781984,"about_ca_topic_score_gemma":0.001902445,"domain_scores_codex":[0.9997038,0.00006140614,0.00005728422,0.00003882356,0.0001022528,0.00003638978],"domain_scores_gemma":[0.9998369,0.00003593098,0.00004346749,0.00002917632,0.00003249761,0.00002186907],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006884512,0.00001670731,0.0001344741,0.00002267166,0.000006494499,0.00003953567,0.0000162066,0.0001755144,0.9987013,0.0001375427,0.00002839961,0.0006522503],"study_design_scores_gemma":[0.00001220096,0.00009427429,0.0006365312,0.000005267754,0.00001383534,0.0001154393,0.0000358129,0.001494175,0.9952406,0.00002986929,0.002314973,0.000007033569],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9767548,0.0008021545,0.01850328,0.0001615004,0.00006375017,0.0001030557,0.002206407,0.0003071097,0.001097746],"genre_scores_gemma":[0.9728014,0.0008737287,0.02057673,0.00006047049,0.000007661152,0.00007425567,0.002934391,0.0001309175,0.002540369],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001781984,"threshold_uncertainty_score":0.003543258,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01468680489239536,"score_gpt":0.2244251379521248,"score_spread":0.2097383330597295,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}