{"id":"W3160834213","doi":"10.1038/s41598-021-89657-8","title":"Validation of reference genes for use in untreated bovine fibroblasts","year":2021,"lang":"en","type":"article","venue":"Scientific Reports","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph; Toronto Zoo","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Reference genes; Normalization (sociology); Database normalization; Gene; Biology; Computational biology; Gene expression; Fibroblast; Bioinformatics; Genetics; Molecular biology; Cell culture; Computer science","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.01099345,0.001698206,0.002479021,0.003231922,0.002289005,0.002529081,0.00177584,0.001588789,0.003702091],"category_scores_gemma":[0.01327269,0.0008871796,0.001312786,0.003980022,0.002429432,0.0009718603,0.001405596,0.001958141,0.002937562],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00147401,"about_ca_system_score_gemma":0.002405802,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00423595,"about_ca_topic_score_gemma":0.01013285,"domain_scores_codex":[0.9864216,0.002535413,0.001650439,0.003418295,0.005249202,0.0007251304],"domain_scores_gemma":[0.9894732,0.003114896,0.001055515,0.001776016,0.004418956,0.0001613968],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002500674,0.0001050019,0.001751559,0.0006130428,0.00003342468,0.0001604261,0.000773997,0.0004678038,0.9801136,0.0007886676,0.0006084655,0.01433395],"study_design_scores_gemma":[0.00002502346,0.0005356183,0.01284903,0.0003489958,0.0001925483,0.0003675333,0.0004814733,0.002351263,0.9416022,0.0008863008,0.04026838,0.00009171717],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.3182059,0.01289592,0.6233534,0.0007793428,0.002067775,0.004508502,0.01984665,0.004003584,0.01433884],"genre_scores_gemma":[0.2267487,0.008751954,0.6934441,0.0009009921,0.0002070368,0.01551591,0.0348076,0.002829926,0.01679382],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.01099345,"threshold_uncertainty_score":0.05813962,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02271987273590581,"score_gpt":0.2846505542117382,"score_spread":0.2619306814758324,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}