{"id":"W3162979399","doi":"10.1021/acschembio.1c00063","title":"Orthogonal Active-Site Labels for Mixed-Linkage endo-β-Glucanases","year":2021,"lang":"en","type":"article","venue":"ACS Chemical Biology","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"Canada's Michael Smith Genome Sciences Centre; University of British Columbia","funders":"Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; University of British Columbia","keywords":"Active site; Glycoside hydrolase; Biochemistry; Hydrolase; Enzyme; Chemistry; Stereochemistry; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003739252,0.0007598042,0.0003223135,0.0002418006,0.0001432205,0.0003117613,0.0005775942,0.0005588084,0.001055941],"category_scores_gemma":[0.0003015467,0.0002454568,0.0002826591,0.0002657576,0.00032933,0.0004180789,0.0003308232,0.0009010011,0.0006004379],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005041139,"about_ca_system_score_gemma":0.0002667694,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005012144,"about_ca_topic_score_gemma":0.0008313701,"domain_scores_codex":[0.9996705,0.00004993899,0.00002166366,0.00009607861,0.00008891557,0.0000729189],"domain_scores_gemma":[0.999823,0.00004475913,0.00005731586,0.00002164303,0.00002896218,0.00002430088],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006655522,0.00003082853,0.00006080189,0.00008359335,0.000005694276,0.00003435254,0.00002145708,0.0001893921,0.9959018,0.0005154505,0.0001034756,0.002986699],"study_design_scores_gemma":[0.00000644036,0.0001081617,0.0001348413,0.000003929977,0.000004138258,0.0000412241,0.00000464876,0.0003300358,0.9971408,0.00003060452,0.002190979,0.000004352171],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8688451,0.006432167,0.1126341,0.0002380035,0.0001383422,0.0002649796,0.001285536,0.000586413,0.009575359],"genre_scores_gemma":[0.9449484,0.004645446,0.04295854,0.0002174475,0.00003105008,0.0002816823,0.001340681,0.00009919069,0.005477436],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001055941,"threshold_uncertainty_score":0.003657699,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01182928980621186,"score_gpt":0.2639193303570889,"score_spread":0.252090040550877,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}