{"id":"W3162979399","doi":"10.1021/acschembio.1c00063","title":"Orthogonal Active-Site Labels for Mixed-Linkage endo-β-Glucanases","year":2021,"lang":"en","type":"article","venue":"ACS Chemical Biology","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"Canada's Michael Smith Genome Sciences Centre; University of British Columbia","funders":"Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; University of British Columbia","keywords":"Active site; Glycoside hydrolase; Biochemistry; Hydrolase; Enzyme; Chemistry; Stereochemistry; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0000633887,0.0001309579,0.0001510958,0.00001842619,0.00005151148,0.00001077863,0.0001011095,0.0002456311,0.00006586772],"category_scores_gemma":[0.0003621496,0.0001279441,0.00008701277,0.00007674877,0.00008356525,0.000003828981,0.000101012,0.00008373815,0.00001552063],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001097865,"about_ca_system_score_gemma":0.00007574631,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000001629796,"about_ca_topic_score_gemma":0.00000861399,"domain_scores_codex":[0.999051,0.00004729854,0.0001761727,0.0004499574,0.000041316,0.0002343047],"domain_scores_gemma":[0.9994205,0.00003237988,0.00006663918,0.0002376262,0.0001719358,0.00007091613],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008267529,0.00006812881,0.0007505489,0.00001367654,0.00004511731,0.000001272666,0.000009362812,4.911427e-7,0.990009,0.0008101305,0.001796114,0.006413461],"study_design_scores_gemma":[0.0004116729,0.00005756555,0.0003999437,0.000002956442,0.00001314824,0.00002224847,0.00001314062,0.00000221142,0.796724,0.0001780588,0.2020494,0.000125672],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9965845,0.0004068754,0.001049056,0.0009383174,0.0003229019,0.0001122518,0.0002599551,0.00002095243,0.0003051929],"genre_scores_gemma":[0.9884989,0.0002261331,0.001077809,0.001269593,0.0007487554,0.00005812966,0.007339851,0.00001852052,0.0007623101],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2002533,"threshold_uncertainty_score":0.521741,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01182928980621186,"score_gpt":0.2639193303570889,"score_spread":0.252090040550877,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}