{"id":"W3163418875","doi":"10.1007/s10529-021-03127-9","title":"Genetic transformation system for Bacillus velezensis NSZ-YBGJ001 and curing of the endogenous plasmid pBV01","year":2021,"lang":"en","type":"article","venue":"Biotechnology Letters","topic":"Biopolymer Synthesis and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":false,"ca_institutions":"Biotechnology Research Institute","funders":"National Natural Science Foundation of China","keywords":"Plasmid; Bacillus subtilis; Transformation (genetics); Biology; Gene; Genetics; Microbiology; Bacteria","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002539706,0.0004944656,0.0004921012,0.0004980578,0.0004003295,0.0003973487,0.0006284904,0.000348744,0.001784836],"category_scores_gemma":[0.0002158498,0.0002807025,0.0003867718,0.0005463772,0.0002745957,0.0001981222,0.0006291794,0.001273293,0.001626844],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006689618,"about_ca_system_score_gemma":0.0007517156,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002015524,"about_ca_topic_score_gemma":0.002161937,"domain_scores_codex":[0.999693,0.000032107,0.00003506635,0.00006405024,0.0001112828,0.00006453935],"domain_scores_gemma":[0.9998966,0.00001831015,0.0000212606,0.00002108865,0.00001676581,0.00002589173],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004416852,0.00006149415,0.0001288745,0.00004892619,0.000003142197,0.00003650959,0.00003454546,0.00006035158,0.996644,0.0004011848,0.0001120721,0.00242468],"study_design_scores_gemma":[0.00002704504,0.0001081083,0.002182683,0.0000151367,0.00001821799,0.0002135334,0.00006590952,0.0009106772,0.9858835,0.0001473989,0.01041162,0.00001620753],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9104834,0.0009887778,0.07227789,0.0005683036,0.0003157071,0.0006152961,0.004492831,0.001361677,0.008896073],"genre_scores_gemma":[0.8997192,0.001048476,0.04515913,0.0001236965,0.00003168997,0.0007390754,0.01854333,0.0005676841,0.03406785],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002015524,"threshold_uncertainty_score":0.005970836,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009834418436949047,"score_gpt":0.1888970297919401,"score_spread":0.1790626113549911,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}