{"id":"W3163874868","doi":"10.17504/protocols.io.s4regv6","title":"Transformation of Acanthamoeba castellanii with Qiagen SuperFect reagent v1","year":2018,"lang":"en","type":"preprint","venue":"","topic":"Legionella and Acanthamoeba research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Dalhousie University","funders":"","keywords":"Transformation (genetics); Plasmid; Reagent; Chemistry; DNA; Computational biology; Microbiology; Biology; Chromatography; Molecular biology; Genetics; Gene; Organic chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003026866,0.0002884033,0.0003124373,0.00009584358,0.00006171448,0.00003282784,0.000398673,0.0004363018,0.0002571211],"category_scores_gemma":[0.00002177067,0.0002132213,0.0001563411,0.00008349021,0.0002148081,0.000003357626,0.0003251976,0.0002541301,0.00002781294],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002399192,"about_ca_system_score_gemma":0.0003648316,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003977109,"about_ca_topic_score_gemma":0.0002515159,"domain_scores_codex":[0.9983667,0.00009941809,0.0003425352,0.0005086245,0.0003530534,0.0003296339],"domain_scores_gemma":[0.9987848,0.0000119995,0.0001217807,0.0006883882,0.0002734211,0.000119608],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001923344,0.0004507115,0.007025521,0.002001043,0.001195937,0.0000440075,0.001495404,0.0006539473,0.9519687,0.0003210112,0.02650636,0.006414046],"study_design_scores_gemma":[0.0007262119,0.00103124,0.0007613017,0.00012473,0.00006310331,0.00004193853,0.0002084071,0.0002148327,0.9806083,0.00005122648,0.01575805,0.0004106058],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9634135,0.0003392196,0.003043827,0.0002028959,0.000147635,0.0006290757,0.00009215108,0.00001732445,0.03211434],"genre_scores_gemma":[0.9954894,0.0007253069,0.0005478067,0.00006905984,0.0003538706,0.00003589211,0.0006736099,0.00004086113,0.002064247],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03207581,"threshold_uncertainty_score":0.8694914,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0206522682816015,"score_gpt":0.2781365624909683,"score_spread":0.2574842942093668,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}