{"id":"W3163874868","doi":"10.17504/protocols.io.s4regv6","title":"Transformation of Acanthamoeba castellanii with Qiagen SuperFect reagent v1","year":2018,"lang":"en","type":"preprint","venue":"","topic":"Legionella and Acanthamoeba research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Dalhousie University","funders":"","keywords":"Transformation (genetics); Plasmid; Reagent; Chemistry; DNA; Computational biology; Microbiology; Biology; Chromatography; Molecular biology; Genetics; Gene; Organic chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001066238,0.001638441,0.002067694,0.002060956,0.0016827,0.001110052,0.001576439,0.001029364,0.009578235],"category_scores_gemma":[0.000789962,0.0009613609,0.001153641,0.00221701,0.000553112,0.0005784222,0.00154611,0.002458286,0.00877801],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00123985,"about_ca_system_score_gemma":0.001258303,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.01179715,"about_ca_topic_score_gemma":0.02018683,"domain_scores_codex":[0.9986377,0.0001645508,0.00009014645,0.0002251684,0.0005497407,0.0003327033],"domain_scores_gemma":[0.9996108,0.00006604679,0.00002932509,0.0001238408,0.00009256214,0.00007734078],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008933674,0.0001011402,0.0002345909,0.0003568035,0.0000138866,0.0001196499,0.00013406,0.000102567,0.9907954,0.000997621,0.002773819,0.004281068],"study_design_scores_gemma":[0.0001028495,0.0002075492,0.005380078,0.00008560507,0.00005180245,0.0005626829,0.0001479134,0.002168713,0.8409244,0.0008606068,0.1494381,0.0000695151],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.505852,0.0131437,0.2688469,0.001597141,0.003252359,0.008045141,0.1102839,0.01621119,0.07276756],"genre_scores_gemma":[0.4356396,0.007893993,0.1924662,0.000689268,0.0002203318,0.004011334,0.2033832,0.003680844,0.1520153],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.01179715,"threshold_uncertainty_score":0.03204238,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0206522682816015,"score_gpt":0.2781365624909683,"score_spread":0.2574842942093668,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}