{"id":"W3169325688","doi":"10.1096/fasebj.2021.35.s1.03116","title":"Transport Mechanism Insights from Newly Identified Substrate Binding Sites in Two SLC4 Transporters","year":2021,"lang":"en","type":"article","venue":"The FASEB Journal","topic":"Ion Transport and Channel Regulation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"Natural Sciences and Engineering Research Council of Canada; National Institutes of Health","keywords":"Chemistry; Symporter; Cotransporter; Biophysics; Mutagenesis; Binding site; Ion transporter; Molecular dynamics; Solute carrier family; Biochemistry; Transporter; Gating; Isothermal titration calorimetry; Transmembrane domain; Membrane; Sodium; Mutation; Biology; Computational chemistry; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001799082,0.0001625836,0.0001628344,0.00006286625,0.0001641641,0.00004549099,0.0001906764,0.00009147667,0.0001457398],"category_scores_gemma":[0.000003614722,0.0001281251,0.0001702838,0.0001527577,0.00004170878,0.00001629708,0.000006393936,0.0002321346,0.000008282743],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000171727,"about_ca_system_score_gemma":0.0001079329,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004270458,"about_ca_topic_score_gemma":0.001331077,"domain_scores_codex":[0.9988793,0.00007028122,0.0003630175,0.0002412748,0.0002129509,0.0002331328],"domain_scores_gemma":[0.9995205,0.000008915787,0.0001093187,0.0001982673,0.00007008752,0.0000929277],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009709501,0.00003876375,0.0061519,0.000004348688,0.00006157438,0.0002417354,0.0007510795,0.000518628,0.9918321,0.0001316978,0.00001484503,0.0001562596],"study_design_scores_gemma":[0.001288313,0.00003154314,0.02971905,0.00003816044,0.00005227077,0.00007856201,0.0008392289,0.0001006485,0.9655107,0.002006893,0.0001563786,0.0001783101],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9939904,0.0005286027,0.004589376,0.0001937061,0.0004164499,0.00007644732,0.00002743578,0.000008744604,0.0001688364],"genre_scores_gemma":[0.9980195,0.0002610445,0.0001489828,0.0001240985,0.0003754811,0.000003418592,0.0006245665,0.00002217528,0.0004207506],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02632143,"threshold_uncertainty_score":0.5224791,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0144081206954918,"score_gpt":0.2370580423914906,"score_spread":0.2226499216959988,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}