{"id":"W3170515163","doi":"10.21769/bioprotoc.4041","title":"Preparation of Doublet Microtubule Fraction for Single Particle Cryo-electron Microscopy","year":2021,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Advanced Electron Microscopy Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"Precursory Research for Embryonic Science and Technology; Natural Sciences and Engineering Research Council of Canada; Japan Society for the Promotion of Science; Canadian Institutes of Health Research; Foundation for Nara Institute of Science and Technology","keywords":"Cilium; Dynein; Microtubule; Flagellum; Tetrahymena; Cryo-electron microscopy; Biophysics; Electron microscope; Ultrastructure; Single particle analysis; Chlamydomonas reinhardtii; Motile cilium; Basal body; Negative stain; Chlamydomonas; Biology; Chemistry; Cell biology; Physics; Anatomy; Optics; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00028276,0.0008059603,0.0006976713,0.000814827,0.0009475464,0.0003199741,0.000774144,0.0005491658,0.003010279],"category_scores_gemma":[0.0002547323,0.000350536,0.0003348895,0.0005842836,0.0002213142,0.0004265275,0.000549208,0.001530255,0.00181556],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003215377,"about_ca_system_score_gemma":0.0004073739,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001369387,"about_ca_topic_score_gemma":0.003338082,"domain_scores_codex":[0.9998154,0.000008102632,0.00002059143,0.00006598821,0.00004276738,0.000047101],"domain_scores_gemma":[0.9997935,0.00002323161,0.0000235389,0.00005617903,0.00006500154,0.00003874108],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004395317,0.00004880154,0.0002945002,0.0001193533,0.00001304488,0.0001541887,0.00006153909,0.0001241817,0.9952896,0.0004428865,0.0004114486,0.00299653],"study_design_scores_gemma":[0.00008459527,0.0005002284,0.02026256,0.0001229168,0.0001488245,0.001174804,0.0002095111,0.005428721,0.906211,0.001230375,0.06456452,0.00006201606],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"protocol","genre_scores_codex":[0.6742969,0.01121193,0.2668298,0.0007782781,0.001090888,0.003105775,0.02052955,0.00235041,0.01980647],"genre_scores_gemma":[0.5686842,0.01054575,0.3319491,0.0005998939,0.0004035783,0.004578258,0.06327298,0.0007833988,0.01918279],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.003010279,"threshold_uncertainty_score":0.01007038,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01548960735618109,"score_gpt":0.411852046235389,"score_spread":0.3963624388792079,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}