{"id":"W3174321621","doi":"10.1101/2021.06.18.448732","title":"Zebrafish Cre/ <i>lox</i> regulated UFlip alleles generated by CRISPR/Cas targeted integration provide cell-type specific conditional gene inactivation","year":2021,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"","keywords":"Biology; Genetics; Gene targeting; Gene; CRISPR; Cre recombinase; Cas9; Reporter gene; Gene expression; Transgene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001809496,0.0007050101,0.000371449,0.0005337007,0.0002917957,0.0003478921,0.0005745375,0.0005556202,0.007179849],"category_scores_gemma":[0.000103581,0.0003422085,0.0003730482,0.0001714373,0.0004939589,0.0002644468,0.0004555273,0.0008375763,0.002639474],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005127396,"about_ca_system_score_gemma":0.0004742495,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00178867,"about_ca_topic_score_gemma":0.002908672,"domain_scores_codex":[0.9997767,0.0000130881,0.0000233196,0.00005374382,0.00008862531,0.00004449319],"domain_scores_gemma":[0.9998435,0.00001480148,0.0000703322,0.00001884282,0.00001734417,0.00003511856],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001506388,0.000004009142,0.00003497548,0.00002348411,0.000001616981,0.00004742107,0.000005281157,0.00003477841,0.998662,0.0001484288,0.0001113527,0.0009115902],"study_design_scores_gemma":[0.00002186567,0.00006510208,0.001469298,0.00001290999,0.00001221275,0.000392187,0.00001237177,0.0008344255,0.9883158,0.00005903861,0.008794429,0.00001036394],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8234219,0.001980724,0.1350624,0.0006577107,0.0003692057,0.0006279287,0.009277769,0.006989646,0.02161288],"genre_scores_gemma":[0.8864775,0.001253874,0.0590657,0.0002507303,0.00004578145,0.0005009483,0.006217175,0.0008566669,0.04533163],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.007179849,"threshold_uncertainty_score":0.024019,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007834032369720684,"score_gpt":0.225982853544397,"score_spread":0.2181488211746763,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}