{"id":"W3174508705","doi":"10.1101/2021.07.01.450725","title":"Programmable Cleavage of Double-stranded DNA by Combined Action of Argonaute CbAgo from <i>Clostridium butyricum</i> and Nuclease Deficient RecBC Helicase from <i>E.coli</i>","year":2021,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"","keywords":"Nuclease; RecBCD; Helicase; DNA; Biology; Molecular biology; Biochemistry; DNA repair; RNA; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001649775,0.0003884221,0.0001852952,0.0001167351,0.00007340113,0.0001672682,0.00019031,0.0001860417,0.0005450984],"category_scores_gemma":[0.0001298684,0.0001172984,0.000255023,0.00008636753,0.0001602861,0.0001125435,0.000213816,0.000317241,0.000121619],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001397244,"about_ca_system_score_gemma":0.0001563051,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006215842,"about_ca_topic_score_gemma":0.0007581489,"domain_scores_codex":[0.9998587,0.00001876931,0.00001204719,0.00003148848,0.00004078174,0.00003821055],"domain_scores_gemma":[0.9998769,0.00002824173,0.0000411792,0.00001367568,0.00001175298,0.00002828062],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000225678,0.00000428782,0.00005639371,0.00001606851,0.000001874125,0.00001588804,0.000003461683,0.00001807117,0.9996012,0.00001202715,0.000003149206,0.000245083],"study_design_scores_gemma":[0.000002902243,0.00008494451,0.0005558828,0.000002036801,0.000003701074,0.00006364787,0.000005825595,0.0001854709,0.9987915,0.000004746988,0.0002974195,0.000001914272],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9958853,0.000569949,0.002843457,0.00002609104,0.00001610648,0.00001826634,0.00009743074,0.00004071586,0.0005026678],"genre_scores_gemma":[0.9962922,0.0001948752,0.002599201,0.00001387857,0.000002213355,0.00001307171,0.0001602991,0.000009758034,0.0007144243],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006215842,"threshold_uncertainty_score":0.001823485,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01007776203258344,"score_gpt":0.2370662326606964,"score_spread":0.226988470628113,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}