{"id":"W3174862079","doi":"10.21769/bioprotoc.4078","title":"Immunofluorescence of GFAP and TNF-α in the Mouse Hypothalamus","year":2021,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Immune Response and Inflammation","field":"Immunology and Microbiology","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Banting and Best Diabetes Centre, University of Toronto; Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs","keywords":"Immunofluorescence; Hypothalamus; Immunostaining; Neuropeptide; Biology; Glial fibrillary acidic protein; Endoplasmic reticulum; Antibody; Cell biology; Pathology; Immunohistochemistry; Neuroscience; Immunology; Medicine; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007021075,0.001061819,0.000503009,0.001620608,0.0005671954,0.0004821709,0.0006309848,0.000860136,0.005346678],"category_scores_gemma":[0.0002786187,0.0005995613,0.0007169166,0.0006101906,0.0006334996,0.0006321945,0.0004978971,0.002310008,0.003050712],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003608824,"about_ca_system_score_gemma":0.0004881195,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005729751,"about_ca_topic_score_gemma":0.001105983,"domain_scores_codex":[0.9993844,0.00005605631,0.00006789376,0.0001968603,0.0001686899,0.0001260466],"domain_scores_gemma":[0.9996179,0.00004635948,0.00007930427,0.00005537518,0.0001135517,0.00008734808],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001232409,0.00002968224,0.00009106017,0.0001804142,0.00001018103,0.0001133243,0.0000624369,0.00003742773,0.9968764,0.0004446114,0.0001702403,0.001861043],"study_design_scores_gemma":[0.00004101247,0.0003479268,0.002829347,0.0001472851,0.00004898099,0.0007702091,0.0000900778,0.0005539213,0.9801297,0.0003498726,0.01467397,0.00001773498],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.601211,0.02709704,0.3059294,0.001175197,0.002321381,0.001804266,0.01237565,0.004933912,0.04315228],"genre_scores_gemma":[0.5464519,0.03020908,0.3298684,0.0008458567,0.0006781512,0.005126284,0.0150324,0.002238258,0.06954972],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.005346678,"threshold_uncertainty_score":0.01788646,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01616027388612319,"score_gpt":0.2740496594804605,"score_spread":0.2578893855943373,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}