{"id":"W3174870876","doi":"10.1128/aem.00647-21","title":"Improved Dynamic Range of a Rhamnose-Inducible Promoter for Gene Expression in <i>Burkholderia</i> spp.","year":2021,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":17,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Manitoba","funders":"Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; Mitacs; Government of Canada","keywords":"Biology; Burkholderia cenocepacia; Promoter; Gene; Genetics; Plasmid; Burkholderia; lac operon; Gene cluster; Mutant; Regulation of gene expression; Reporter gene; Gene expression; Molecular biology; Bacteria; Virulence","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004548493,0.000420977,0.0003747493,0.0002367675,0.0001782297,0.0005843839,0.0005223679,0.0003917401,0.001106073],"category_scores_gemma":[0.0005089741,0.0002468191,0.0003658852,0.0002544185,0.0002661808,0.0004295079,0.0005721503,0.001002758,0.0007683178],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005532833,"about_ca_system_score_gemma":0.0003873105,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008978097,"about_ca_topic_score_gemma":0.001401332,"domain_scores_codex":[0.9995098,0.00007601185,0.00006228331,0.0001303895,0.0001432167,0.00007830807],"domain_scores_gemma":[0.9996331,0.0001040749,0.00009340254,0.00006046859,0.00006067939,0.00004823603],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003076065,0.00001856713,0.00005904915,0.00002287656,0.00000181418,0.00001752154,0.00001761097,0.0001120573,0.9982339,0.00009612672,0.00003398937,0.001355738],"study_design_scores_gemma":[0.000007163364,0.00006051469,0.0004404524,0.000007718117,0.000006932339,0.00008175005,0.00001732562,0.001197233,0.995454,0.0000517972,0.002662655,0.00001253512],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9001028,0.001632855,0.09026973,0.0005914634,0.0001491323,0.0001341112,0.001090336,0.001396773,0.004632792],"genre_scores_gemma":[0.9635633,0.0006159177,0.03002276,0.0001607882,0.00001946556,0.0001248952,0.001411363,0.0003296459,0.003751825],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001106073,"threshold_uncertainty_score":0.004014373,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003497529150515461,"score_gpt":0.2155129047185706,"score_spread":0.2120153755680551,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}