{"id":"W3174870876","doi":"10.1128/aem.00647-21","title":"Improved Dynamic Range of a Rhamnose-Inducible Promoter for Gene Expression in <i>Burkholderia</i> spp.","year":2021,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":17,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Manitoba","funders":"Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; Mitacs; Government of Canada","keywords":"Biology; Burkholderia cenocepacia; Promoter; Gene; Genetics; Plasmid; Burkholderia; lac operon; Gene cluster; Mutant; Regulation of gene expression; Reporter gene; Gene expression; Molecular biology; Bacteria; Virulence","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00005463254,0.0001175792,0.0001564866,0.00001971396,0.00002109792,0.000003081428,0.00005400163,0.0001469505,0.00001625862],"category_scores_gemma":[0.000003021582,0.0001160066,0.00003982892,0.00002062614,0.00005932183,0.000001289619,0.0001085336,0.0000457053,9.083697e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000009048568,"about_ca_system_score_gemma":0.000009993571,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000002364459,"about_ca_topic_score_gemma":0.000009846057,"domain_scores_codex":[0.9993126,0.0000110496,0.000160183,0.0003236394,0.00001322442,0.0001793236],"domain_scores_gemma":[0.9997591,0.000006919687,0.00003436187,0.0001679529,0.000002575213,0.0000290736],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004990732,0.0000695803,0.000484303,0.00002969523,0.00001351244,0.00000105655,0.00004421398,0.00003012507,0.99837,0.000002057819,0.00003328028,0.0008723037],"study_design_scores_gemma":[0.001171135,0.00009942272,0.001229173,0.000006864435,0.00000848086,0.00003330241,0.0001081609,0.00004463122,0.9947548,0.00002313775,0.002391471,0.0001294284],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9942585,0.001622355,0.003636401,0.00002070549,0.00005648681,0.0002483933,0.00009780674,0.000003052992,0.00005633724],"genre_scores_gemma":[0.9950262,0.0003784024,0.003787125,0.00006842726,0.00002752625,0.0000537048,0.0005140444,0.00001686414,0.0001277048],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003615172,"threshold_uncertainty_score":0.4730614,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003497529150515461,"score_gpt":0.2155129047185706,"score_spread":0.2120153755680551,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}