{"id":"W3176333140","doi":"10.3390/genes12070960","title":"Current Practices for Reference Gene Selection in RT-qPCR of Aspergillus: Outlook and Recommendations for the Future","year":2021,"lang":"en","type":"review","venue":"Genes","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":25,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Reference genes; Gene; Biology; Gene expression; Aspergillus; Computational biology; Real-time polymerase chain reaction; Gene expression profiling; Genetics; Bioinformatics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.01193473,0.001230337,0.00307624,0.004872485,0.0005226787,0.002766195,0.002530428,0.0024959,0.004311911],"category_scores_gemma":[0.01258485,0.0007180527,0.002181939,0.004377747,0.001242577,0.004346994,0.001082809,0.002930282,0.003084167],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00197115,"about_ca_system_score_gemma":0.007303165,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002734265,"about_ca_topic_score_gemma":0.004616068,"domain_scores_codex":[0.9969619,0.0008609187,0.0008160289,0.0003747961,0.0008573869,0.0001289827],"domain_scores_gemma":[0.9844435,0.009319616,0.001324427,0.0003616381,0.004268027,0.0002826289],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"design_other","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0001275768,0.00007742701,0.0005043774,0.1080099,0.0002406547,0.0003108697,0.0002487208,0.0004545086,0.003583319,0.006749408,0.03370061,0.8459926],"study_design_scores_gemma":[0.00003024351,0.0001302475,0.001133296,0.04738544,0.0009176105,0.000676298,0.0002907446,0.0002037573,0.001640039,0.005793001,0.9417284,0.00007094099],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"review","genre_gemma":"review","genre_scores_codex":[0.0001205224,0.9952328,0.001039865,0.0021398,0.000582242,0.00002884665,0.00009857008,0.0000343479,0.0007230569],"genre_scores_gemma":[0.0007927378,0.9924314,0.004523618,0.001143423,0.0002542466,0.00009688851,0.0002221678,0.00001489142,0.0005205522],"genre_candidate":"review","genre_consensus":"review","teacher_disagreement_score":0.01193473,"threshold_uncertainty_score":0.06311762,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.08203900784826501,"score_gpt":0.4236353066910308,"score_spread":0.3415962988427658,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}