{"id":"W3176799446","doi":"10.1002/pro.4150","title":"Heat treatment of thioredoxin fusions increases the purity of α‐helical transmembrane protein constructs","year":2021,"lang":"en","type":"article","venue":"Protein Science","topic":"Lipid Membrane Structure and Behavior","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hospital for Sick Children","funders":"H2020 Marie Skłodowska-Curie Actions; European Social Fund; Canadian Institutes of Health Research; Horizon 2020 Framework Programme; Technische Universität Dresden; Cystic Fibrosis Canada; Boehringer Ingelheim Stiftung; European Commission; University of Cambridge","keywords":"Thioredoxin; Transmembrane protein; Membrane protein; Thermostability; Chemistry; Protein purification; Transmembrane domain; Biochemistry; Fusion protein; Chromatography; Membrane; Biology; Enzyme; Recombinant DNA; Receptor","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002070856,0.0001337074,0.0001983247,0.00003258565,0.0001464158,0.00001723429,0.0003113234,0.00007511302,0.00006439062],"category_scores_gemma":[0.0001638066,0.00008289466,0.0001097337,0.0003572474,0.001168278,0.000007986398,0.00008971185,0.00005409124,0.000001006429],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001402431,"about_ca_system_score_gemma":0.0007407384,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001771834,"about_ca_topic_score_gemma":0.00009513204,"domain_scores_codex":[0.9987439,0.00008417937,0.0002494121,0.0003689573,0.0003253062,0.0002282355],"domain_scores_gemma":[0.9990682,0.00001128903,0.00005034472,0.0005388606,0.0002391332,0.00009216394],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003271916,0.0001918216,0.0002714929,0.00002710188,0.00000896838,0.000003911167,0.00009258322,0.000001988659,0.9951842,0.0002986341,0.000002842311,0.003883765],"study_design_scores_gemma":[0.0004471794,0.0003521714,0.001373431,0.00002916545,0.00002394016,0.00004567529,0.0001054218,0.00001103275,0.9967011,0.00003968443,0.0007763183,0.00009488947],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9982005,0.0003579925,0.00003331347,0.0002975312,0.00005936802,0.0006529745,0.00005481087,0.000005751959,0.0003377883],"genre_scores_gemma":[0.9985266,0.000049841,0.001064386,0.00002915799,0.00008554718,0.00007538988,0.00001443388,0.000006422284,0.0001481913],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003788876,"threshold_uncertainty_score":0.4304571,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01317821358314882,"score_gpt":0.2668346954728064,"score_spread":0.2536564818896576,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}