{"id":"W3176799446","doi":"10.1002/pro.4150","title":"Heat treatment of thioredoxin fusions increases the purity of α‐helical transmembrane protein constructs","year":2021,"lang":"en","type":"article","venue":"Protein Science","topic":"Lipid Membrane Structure and Behavior","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hospital for Sick Children","funders":"H2020 Marie Skłodowska-Curie Actions; European Social Fund; Canadian Institutes of Health Research; Horizon 2020 Framework Programme; Technische Universität Dresden; Cystic Fibrosis Canada; Boehringer Ingelheim Stiftung; European Commission; University of Cambridge","keywords":"Thioredoxin; Transmembrane protein; Membrane protein; Thermostability; Chemistry; Protein purification; Transmembrane domain; Biochemistry; Fusion protein; Chromatography; Membrane; Biology; Enzyme; Recombinant DNA; Receptor","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008319241,0.000559823,0.0004195929,0.000346286,0.0003313449,0.000537206,0.0003540753,0.0004013077,0.0008866586],"category_scores_gemma":[0.0006049166,0.0002665899,0.000361648,0.0003959649,0.0004668585,0.0004240674,0.0005154742,0.001161724,0.0007022059],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004142867,"about_ca_system_score_gemma":0.0002738835,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004258994,"about_ca_topic_score_gemma":0.0006614919,"domain_scores_codex":[0.9993734,0.0001386553,0.0000687763,0.0001431899,0.0001980605,0.00007801868],"domain_scores_gemma":[0.9994736,0.0001418337,0.0001216537,0.0001022422,0.00011028,0.00005036142],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000231095,0.00001089577,0.00005550844,0.00002034294,0.000005355503,0.00002026046,0.00002543536,0.00003456099,0.9991616,0.00007091063,0.00003634293,0.0005356002],"study_design_scores_gemma":[0.000002587789,0.00003352351,0.0003875229,0.000003820833,0.000006194228,0.00004636872,0.000007447491,0.0003021965,0.9978482,0.00002494341,0.001333974,0.000003170214],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9101171,0.00159,0.08381787,0.0004759167,0.0001018708,0.0002042672,0.0008395868,0.0008049647,0.002048418],"genre_scores_gemma":[0.934378,0.002440223,0.05363165,0.0002359661,0.00003344411,0.000298436,0.002916121,0.0005044666,0.005561769],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008866586,"threshold_uncertainty_score":0.004399657,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01317821358314882,"score_gpt":0.2668346954728064,"score_spread":0.2536564818896576,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}