{"id":"W3179690541","doi":"10.1093/biomethods/bpab004","title":"Optimization and biological validation of an <i>in vitro</i> assay using the transfected Dm28c/pLacZ <i>Trypanosoma cruzi</i> strain","year":2021,"lang":"en","type":"article","venue":"Biology Methods and Protocols","topic":"Trypanosoma species research and implications","field":"Medicine","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"","keywords":"Trypanosoma cruzi; Nifurtimox; Amastigote; Benznidazole; Bioassay; In vitro; Strain (injury); In vitro toxicology; Chagas disease; Molecular biology; Biology; Chemistry; Microbiology; Biochemistry; Virology; Parasite hosting; Leishmania","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002489053,0.0001166305,0.0003115465,0.0000692957,0.000100881,0.00002404281,0.000067261,0.0002018234,0.00005572051],"category_scores_gemma":[0.0005656191,0.00007510481,0.00004130769,0.0003174428,0.0002953432,0.00006872709,0.00003993147,0.000194389,1.507193e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001611793,"about_ca_system_score_gemma":0.0001300868,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001763272,"about_ca_topic_score_gemma":0.000002715799,"domain_scores_codex":[0.9972491,0.001847379,0.0003151274,0.0003085769,0.00006988639,0.0002098833],"domain_scores_gemma":[0.9989517,0.0004977786,0.00007263556,0.0002419301,0.0001375941,0.00009831433],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002489536,0.0001158083,0.001278286,0.00004442978,0.00001916462,0.000002962586,0.00007197398,0.00002128315,0.9658364,0.0005419276,0.000002909859,0.03181586],"study_design_scores_gemma":[0.002090496,0.0007978415,0.0654511,0.00006440179,0.0000294533,0.000193252,0.0001895182,0.006055043,0.9208677,0.00150407,0.002615413,0.0001417096],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.5311942,0.000388508,0.4307376,0.001866446,0.00003602838,0.03502117,0.0002357821,0.00004821007,0.0004721063],"genre_scores_gemma":[0.1763003,0.0001505601,0.8105677,0.000404682,0.0001129713,0.01210186,0.0003141487,0.00002045467,0.00002725409],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.3798301,"threshold_uncertainty_score":0.3062685,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1352993334674543,"score_gpt":0.483322546083806,"score_spread":0.3480232126163517,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}