{"id":"W3182887434","doi":"10.1016/j.ijbiomac.2021.07.009","title":"Site-specific internal protein labeling through trans-splicing","year":2021,"lang":"en","type":"article","venue":"International Journal of Biological Macromolecules","topic":"Chemical Synthesis and Analysis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":false,"ca_institutions":"Dalhousie University","funders":"Science and Technology Commission of Shanghai Municipality; National Natural Science Foundation of China","keywords":"Protein splicing; Protein tag; Biochemistry; Chemistry; Peptide; dnaB helicase; FLAG-tag; Maltose-binding protein; Intein; RNA splicing; Linker; Recombinant DNA; Gene; Fusion protein; RNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001018125,0.0008964583,0.0005373381,0.0004164278,0.0005013575,0.0007194047,0.0007549126,0.000735542,0.001971973],"category_scores_gemma":[0.0005547135,0.0004999061,0.0008858551,0.0004713662,0.0007999425,0.0005096552,0.0008358709,0.001921962,0.002205427],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004529631,"about_ca_system_score_gemma":0.0005291008,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003916727,"about_ca_topic_score_gemma":0.0007443321,"domain_scores_codex":[0.9992508,0.0001902078,0.00005092986,0.0002481249,0.0001504421,0.0001093585],"domain_scores_gemma":[0.9992253,0.0001800903,0.0001192907,0.0002682243,0.0001386585,0.0000685367],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000456038,0.00001370658,0.00006155718,0.00002869278,0.000004567137,0.00004529739,0.00003668522,0.00006242479,0.9969331,0.0007942105,0.00007692292,0.001897277],"study_design_scores_gemma":[0.000004114529,0.0000297204,0.00008237208,0.000001818898,0.000007202873,0.00006755722,0.000006367507,0.0002855147,0.9980636,0.0000784378,0.00137011,0.000003140467],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6177166,0.001120719,0.3634012,0.0005725736,0.0003657584,0.0003881374,0.001220959,0.001793075,0.01342103],"genre_scores_gemma":[0.8413504,0.001366734,0.1331517,0.0002459664,0.00007340515,0.0002969466,0.003231843,0.001144095,0.01913892],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001971973,"threshold_uncertainty_score":0.006596863,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02027320313188802,"score_gpt":0.2709921314850607,"score_spread":0.2507189283531727,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}