{"id":"W3182887434","doi":"10.1016/j.ijbiomac.2021.07.009","title":"Site-specific internal protein labeling through trans-splicing","year":2021,"lang":"en","type":"article","venue":"International Journal of Biological Macromolecules","topic":"Chemical Synthesis and Analysis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":false,"ca_institutions":"Dalhousie University","funders":"Science and Technology Commission of Shanghai Municipality; National Natural Science Foundation of China","keywords":"Protein splicing; Protein tag; Biochemistry; Chemistry; Peptide; dnaB helicase; FLAG-tag; Maltose-binding protein; Intein; RNA splicing; Linker; Recombinant DNA; Gene; Fusion protein; RNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001916776,0.0001513861,0.0002369065,0.00003557616,0.00003935994,0.00007722727,0.0004176706,0.0001399872,0.000263917],"category_scores_gemma":[0.0002630566,0.00011346,0.0003937481,0.00007676376,0.00008366132,0.000007607455,0.0001355622,0.0001825283,0.000009240369],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002809659,"about_ca_system_score_gemma":0.00003894635,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000007198846,"about_ca_topic_score_gemma":0.000005094561,"domain_scores_codex":[0.9986765,0.00009537704,0.000499783,0.0002631957,0.0002865757,0.0001786064],"domain_scores_gemma":[0.9990268,0.00003645186,0.0002355449,0.0001272764,0.0004957466,0.0000781212],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001479295,0.0001383576,0.00107012,0.000002817143,0.0002080146,0.000243193,0.00002541538,0.00002704414,0.9909998,0.0002769097,0.0002303005,0.006630068],"study_design_scores_gemma":[0.000443297,0.0001284378,0.0002995148,0.00008589695,0.00001570156,0.0003224653,0.00007988063,0.00001111205,0.9519557,0.0008562137,0.04565281,0.0001489699],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9795477,0.002464493,0.01569799,0.001161925,0.0001886995,0.00003341513,0.00002464634,0.000005839861,0.0008752901],"genre_scores_gemma":[0.9905853,0.001206718,0.006846931,0.000464501,0.0006981554,0.000002533518,0.00005100134,0.00001166188,0.0001331957],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.04542251,"threshold_uncertainty_score":0.4626765,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02027320313188802,"score_gpt":0.2709921314850607,"score_spread":0.2507189283531727,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}