{"id":"W3183452172","doi":"10.1021/jacs.1c04194","title":"<i>O</i>-Linked-<i>N</i>-Acetylglucosaminylation of the RNA-Binding Protein EWS N-Terminal Low Complexity Region Reduces Phase Separation and Enhances Condensate Dynamics","year":2021,"lang":"en","type":"article","venue":"Journal of the American Chemical Society","topic":"RNA Research and Splicing","field":"Biochemistry, Genetics and Molecular Biology","cited_by":58,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hospital for Sick Children; University of Toronto","funders":"National Cancer Institute; Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; University of Toronto; Canadian Cancer Society; Canada Research Chairs; Children's Cancer Foundation","keywords":"Chemistry; Biophysics; Fluorescence recovery after photobleaching; RNA; Intrinsically disordered proteins; Stress granule; Organelle; Biochemistry; Translation (biology); Messenger RNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002580497,0.00009787515,0.0002104658,0.000007023614,0.000119327,0.00002888623,0.0002261924,0.00005619457,0.000001708656],"category_scores_gemma":[0.0001659825,0.00006234075,0.0002651209,0.0001651837,0.000576783,0.00001255024,0.0001477371,0.0002374707,1.004908e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005209941,"about_ca_system_score_gemma":0.0001338474,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000106478,"about_ca_topic_score_gemma":0.000005104909,"domain_scores_codex":[0.9990296,0.0001186504,0.0003123015,0.0001453825,0.0002400163,0.0001539939],"domain_scores_gemma":[0.9988437,0.00002338866,0.0007050948,0.0001808402,0.0001861354,0.00006087213],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000113562,0.00006474556,0.0003079725,0.0000262312,0.00004881063,0.000001594202,0.00006946439,0.000005556054,0.9971482,0.00001334156,0.0002911268,0.001909371],"study_design_scores_gemma":[0.0005202774,0.0001320075,0.0003508396,0.00007775742,0.00002110339,0.0001081971,0.0003938658,0.0008896854,0.997229,0.00008466638,0.0001202035,0.00007234057],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9979846,0.0001644661,0.0004234156,0.001228841,0.00004909716,0.000109296,0.000009359007,0.000001645933,0.00002920434],"genre_scores_gemma":[0.9978811,0.0001823276,0.001381619,0.0001384743,0.0001365688,0.000002247775,0.000008884476,0.000007929898,0.0002608549],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00183703,"threshold_uncertainty_score":0.2542182,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02095239508301464,"score_gpt":0.3186067006579462,"score_spread":0.2976543055749315,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}