{"id":"W3183452172","doi":"10.1021/jacs.1c04194","title":"<i>O</i>-Linked-<i>N</i>-Acetylglucosaminylation of the RNA-Binding Protein EWS N-Terminal Low Complexity Region Reduces Phase Separation and Enhances Condensate Dynamics","year":2021,"lang":"en","type":"article","venue":"Journal of the American Chemical Society","topic":"RNA Research and Splicing","field":"Biochemistry, Genetics and Molecular Biology","cited_by":58,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hospital for Sick Children; University of Toronto","funders":"National Cancer Institute; Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; University of Toronto; Canadian Cancer Society; Canada Research Chairs; Children's Cancer Foundation","keywords":"Chemistry; Biophysics; Fluorescence recovery after photobleaching; RNA; Intrinsically disordered proteins; Stress granule; Organelle; Biochemistry; Translation (biology); Messenger RNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001066168,0.0002923367,0.0001741535,0.00006279314,0.00008810282,0.000212497,0.000173075,0.0001750393,0.001006619],"category_scores_gemma":[0.0001428735,0.00009915765,0.0002859803,0.00009499358,0.0002155853,0.0001715204,0.0001523601,0.0004118501,0.0002495835],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002488256,"about_ca_system_score_gemma":0.0001527175,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006682379,"about_ca_topic_score_gemma":0.0007500405,"domain_scores_codex":[0.999902,0.00001594759,0.00001307804,0.00002303073,0.00002162796,0.00002434259],"domain_scores_gemma":[0.9998944,0.00001820399,0.00004191131,0.00001335369,0.000008873931,0.00002323719],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005598895,0.000008207312,0.00005558075,0.00001247875,0.000003088654,0.00000879395,0.000003653136,0.00002106115,0.9995667,0.00002738426,0.00001243847,0.0002246568],"study_design_scores_gemma":[0.000004014779,0.00005694147,0.001144285,0.000001535073,0.000004784562,0.00003762198,0.000005346741,0.000255173,0.9977514,0.000009050397,0.0007280741,0.000001785611],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9960793,0.0004524647,0.002116154,0.00007506476,0.00001835033,0.00001340101,0.0002249482,0.00004901767,0.0009712592],"genre_scores_gemma":[0.9935644,0.0003857272,0.003060672,0.00009293586,0.000007571527,0.00002189392,0.0009091839,0.00004187258,0.001915683],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001006619,"threshold_uncertainty_score":0.003367543,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02095239508301464,"score_gpt":0.3186067006579462,"score_spread":0.2976543055749315,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}