{"id":"W3184577537","doi":"10.1002/btpr.3193","title":"Preventing pyruvate kinase muscle expression in <scp>Chinese</scp> hamster ovary cells curbs lactogenic behavior by altering glycolysis, gating pyruvate generation, and increasing pyruvate flux into the <scp>TCA</scp> cycle","year":2021,"lang":"en","type":"article","venue":"Biotechnology Progress","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"Microsemi (Canada)","funders":"","keywords":"PKM2; Chinese hamster ovary cell; Pyruvate kinase; Pyruvate dehydrogenase kinase; Citric acid cycle; Pyruvate carboxylase; Flux (metallurgy); Biochemistry; Glycolysis; Pyruvate dehydrogenase phosphatase; Chemistry; Biology; Cell biology; Metabolism; Enzyme; Receptor","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002704008,0.0007000536,0.0005120907,0.0004294221,0.0003558119,0.0005994675,0.0005559908,0.0004290921,0.001751401],"category_scores_gemma":[0.0001840522,0.000325632,0.0006025476,0.0004332312,0.0004130099,0.000329391,0.000492981,0.001020019,0.0009182317],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006131252,"about_ca_system_score_gemma":0.0004632297,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001999316,"about_ca_topic_score_gemma":0.002688921,"domain_scores_codex":[0.9995579,0.00004748702,0.00008331364,0.0001006188,0.0001315732,0.00007914998],"domain_scores_gemma":[0.9996414,0.00004973535,0.0001282738,0.00007957652,0.00004476918,0.00005627075],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003363371,0.00001427774,0.0001154502,0.00002250622,0.000005277172,0.00003517761,0.0000118634,0.00002197067,0.9992378,0.00005406602,0.0000404303,0.0004077288],"study_design_scores_gemma":[0.00001299137,0.00009098674,0.003092307,0.000005555035,0.0000227317,0.0001926737,0.00002904114,0.0006997514,0.991684,0.00002270544,0.004140285,0.000006957827],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9840469,0.001242748,0.008528471,0.0004537043,0.0001632252,0.0001066615,0.002548553,0.0004132804,0.002496556],"genre_scores_gemma":[0.9659581,0.001667858,0.01269972,0.0002732782,0.00004218002,0.0002491408,0.005867114,0.0002951963,0.01294752],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001999316,"threshold_uncertainty_score":0.005859017,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006598423537279578,"score_gpt":0.2481338526120638,"score_spread":0.2415354290747842,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}