{"id":"W3184577537","doi":"10.1002/btpr.3193","title":"Preventing pyruvate kinase muscle expression in <scp>Chinese</scp> hamster ovary cells curbs lactogenic behavior by altering glycolysis, gating pyruvate generation, and increasing pyruvate flux into the <scp>TCA</scp> cycle","year":2021,"lang":"en","type":"article","venue":"Biotechnology Progress","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"Microsemi (Canada)","funders":"","keywords":"PKM2; Chinese hamster ovary cell; Pyruvate kinase; Pyruvate dehydrogenase kinase; Citric acid cycle; Pyruvate carboxylase; Flux (metallurgy); Biochemistry; Glycolysis; Pyruvate dehydrogenase phosphatase; Chemistry; Biology; Cell biology; Metabolism; Enzyme; Receptor","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0005247926,0.0005068204,0.0004045531,0.0001951013,0.0006826305,0.0002370066,0.0004490094,0.0007529017,0.00001026405],"category_scores_gemma":[0.000568114,0.0004295196,0.0001818034,0.0006132093,0.0003647483,0.00005434967,0.001179289,0.0004924186,0.000007669881],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004717275,"about_ca_system_score_gemma":0.00009380639,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00007874241,"about_ca_topic_score_gemma":0.00008784431,"domain_scores_codex":[0.996776,0.0003997527,0.0006725324,0.001138437,0.0002563905,0.0007568793],"domain_scores_gemma":[0.9983129,0.0001102835,0.0004076095,0.0008559346,0.0001487144,0.0001645801],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000007948592,0.0002411661,0.03455382,0.00007193725,0.00005594826,0.0000557339,0.0003035848,0.00007394449,0.954906,0.000007638294,0.001169767,0.0085525],"study_design_scores_gemma":[0.000859876,0.0001446747,0.006440124,0.0001548987,0.00006520758,0.0000304306,0.0005003864,0.001024993,0.9829104,0.00009674647,0.007617247,0.0001550109],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9845093,0.0137684,0.0003294774,0.0001571374,0.0003909107,0.0006134291,0.00004061821,0.0001407175,0.00004997299],"genre_scores_gemma":[0.9955325,0.0009461813,0.001898756,0.0002586329,0.0002924526,0.000399304,0.0003236793,0.00008877746,0.0002597736],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0281137,"threshold_uncertainty_score":0.9998156,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006598423537279578,"score_gpt":0.2481338526120638,"score_spread":0.2415354290747842,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}