{"id":"W3184830572","doi":"10.3791/62510","title":"Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System","year":2021,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":24,"is_retracted":false,"has_abstract":true,"ca_institutions":"Structural Genomics Consortium; University of Toronto","funders":"Innovative Medicines Initiative; Ontario Genomics Institute; European Federation of Pharmaceutical Industries and Associations; Merck KGaA; Ontario Genomics; Genome Canada; Wellcome Trust; Genentech; Bayer; Pfizer","keywords":"Sf9; Recombinant DNA; Biology; Autographa californica; Spodoptera; Shuttle vector; Biochemistry; Arginine; Cell biology; Computational biology; Amino acid; Vector (molecular biology); Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008012853,0.000728278,0.0006933556,0.0008756517,0.0006413623,0.000836838,0.0008601229,0.0006367526,0.001730382],"category_scores_gemma":[0.0004266154,0.0005622732,0.000551382,0.000930602,0.0003539413,0.0005202169,0.0005613672,0.001759443,0.002357458],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007472716,"about_ca_system_score_gemma":0.0005568216,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001237698,"about_ca_topic_score_gemma":0.001630349,"domain_scores_codex":[0.9993728,0.00009855625,0.0001028885,0.000135594,0.0002255769,0.00006466825],"domain_scores_gemma":[0.9998215,0.00003263457,0.0000371295,0.00003906472,0.00004280787,0.00002683167],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003718033,0.00003576681,0.0001631452,0.000169085,0.0000138002,0.00008154519,0.00004404435,0.0001465877,0.9936878,0.0006137379,0.0005556291,0.004451635],"study_design_scores_gemma":[0.00004262767,0.0001967701,0.001490835,0.00003702341,0.0000498443,0.0005029135,0.00003496125,0.003112982,0.9420331,0.0003054787,0.05216195,0.00003148973],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.4543945,0.01314658,0.4823658,0.001089137,0.001006051,0.003892772,0.01933392,0.006961943,0.01780927],"genre_scores_gemma":[0.4753939,0.01186196,0.4346379,0.0003957856,0.0001433201,0.001775247,0.05182292,0.001136216,0.02283263],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.001730382,"threshold_uncertainty_score":0.005788684,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0291386866168961,"score_gpt":0.3906563522674418,"score_spread":0.3615176656505457,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}