{"id":"W3184830572","doi":"10.3791/62510","title":"Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System","year":2021,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":24,"is_retracted":false,"has_abstract":true,"ca_institutions":"Structural Genomics Consortium; University of Toronto","funders":"Innovative Medicines Initiative; Ontario Genomics Institute; European Federation of Pharmaceutical Industries and Associations; Merck KGaA; Ontario Genomics; Genome Canada; Wellcome Trust; Genentech; Bayer; Pfizer","keywords":"Sf9; Recombinant DNA; Biology; Autographa californica; Spodoptera; Shuttle vector; Biochemistry; Arginine; Cell biology; Computational biology; Amino acid; Vector (molecular biology); Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000239099,0.0001304676,0.0002266871,0.00004132011,0.0001351712,0.0000266545,0.0001460078,0.00008813128,0.00002939807],"category_scores_gemma":[0.0001334924,0.00008550511,0.0002109186,0.0001115461,0.00004410107,0.00001347105,0.0001027763,0.00008847971,0.000001055305],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004488317,"about_ca_system_score_gemma":0.0001781144,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000009068661,"about_ca_topic_score_gemma":5.129829e-7,"domain_scores_codex":[0.9986253,0.0002678849,0.0004922205,0.0001832052,0.0002862853,0.0001451447],"domain_scores_gemma":[0.9986412,0.000006949188,0.0006030292,0.0002878828,0.0003851231,0.0000758507],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003385007,0.0003210802,0.0003312264,0.00007059723,0.00009236403,0.00001083837,0.0001543749,0.00009389854,0.9962679,0.00002159574,0.002196977,0.0001006113],"study_design_scores_gemma":[0.0008330519,0.0003784802,0.0001811252,0.0003985599,0.00003581364,0.0001790272,0.0007710432,0.00003363447,0.9938402,0.000009397036,0.003241704,0.0000979284],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9915496,0.00454992,0.001404562,0.00003311654,0.002165182,0.0002054489,0.000002460057,0.000004714038,0.00008496649],"genre_scores_gemma":[0.9976678,0.0001741322,0.001432923,0.00003353168,0.0004250612,0.00001318768,0.000005676266,0.00002000652,0.0002276631],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.006118188,"threshold_uncertainty_score":0.3486797,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0291386866168961,"score_gpt":0.3906563522674418,"score_spread":0.3615176656505457,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}