{"id":"W3191855145","doi":"10.1101/2021.08.10.455711","title":"Cryo-EM reveals disrupted human p97 allosteric activation by disease mutations and inhibitor binding","year":2021,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Endoplasmic Reticulum Stress and Disease","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Biological and Environmental Research; Office of Science; National Institutes of Health; Oregon Health and Science University; U.S. Department of Energy","keywords":"Allosteric regulation; Mutant; Biology; Cell biology; Drug discovery; Nucleotide; Cyclic nucleotide-binding domain; Mutation; Biochemistry; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001451566,0.000237433,0.0001518358,0.0001417801,0.0003203783,0.0002838966,0.0004708965,0.0005721955,0.002350109],"category_scores_gemma":[0.0001652653,0.0001737692,0.0001868657,0.0001514564,0.0003099618,0.0001750018,0.0003008231,0.0007782933,0.0005769336],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000414403,"about_ca_system_score_gemma":0.0001220158,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002551519,"about_ca_topic_score_gemma":0.00169979,"domain_scores_codex":[0.9999429,0.000008180787,0.000003459302,0.00001158375,0.00001722927,0.00001661477],"domain_scores_gemma":[0.999917,0.000029207,0.00001666836,0.00001139562,0.00001021085,0.00001543864],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006538844,0.0001206178,0.002464213,0.00008169861,0.00004132104,0.001660127,0.0001512917,0.001543708,0.9887449,0.0007126162,0.001209906,0.002615538],"study_design_scores_gemma":[0.0001084231,0.0002367125,0.02742252,0.00004384575,0.000050107,0.003580397,0.0002848262,0.01589277,0.9442627,0.0003831133,0.007710044,0.00002455846],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.993919,0.0005573218,0.002053918,0.0002079359,0.00002240851,0.000006730435,0.0004959997,0.0000558531,0.002680835],"genre_scores_gemma":[0.996217,0.0003166976,0.001221655,0.00008804075,0.000004961224,0.000008504106,0.0007156775,0.00004686939,0.00138072],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002551519,"threshold_uncertainty_score":0.007861912,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008631252659588085,"score_gpt":0.2321483990155494,"score_spread":0.2235171463559613,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}