{"id":"W3193267775","doi":"10.7554/elife.67075","title":"Precise optical control of gene expression in C elegans using improved genetic code expansion and Cre recombinase","year":2021,"lang":"en","type":"article","venue":"eLife","topic":"Genetics, Aging, and Longevity in Model Organisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":30,"is_retracted":false,"has_abstract":true,"ca_institutions":"Discovery Centre","funders":"H2020 European Research Council; Medical Research Council Canada; Louis-Jeantet Foundation; University of Edinburgh; Wellcome; Royal Society; National Institutes of Health; University of Cambridge; Wellcome Trust","keywords":"Optogenetics; Caenorhabditis elegans; Recombinase; Cre recombinase; Biology; Synthetic biology; Gene; Genetic code; Cell biology; Genetics; Site-specific recombination; Neuroscience; Computational biology; Transgene; Recombination; Genetically modified mouse","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002676458,0.0003639589,0.0001976728,0.0003743889,0.0002337419,0.000294859,0.0004050734,0.0003502569,0.000751117],"category_scores_gemma":[0.0002670338,0.0002371432,0.0002538225,0.0001419241,0.0005077805,0.000320077,0.0005130683,0.000723297,0.0002477312],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004284457,"about_ca_system_score_gemma":0.0003947916,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001199592,"about_ca_topic_score_gemma":0.002641954,"domain_scores_codex":[0.9997353,0.00002152648,0.00003110179,0.0000645805,0.0001080299,0.000039552],"domain_scores_gemma":[0.9997397,0.00005170224,0.0001022849,0.00004715163,0.0000267996,0.00003240465],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001176545,0.000007897537,0.00005394785,0.00001200841,0.000001344721,0.00002141974,0.000006815125,0.0001586535,0.9979432,0.0002827987,0.0000204081,0.001479768],"study_design_scores_gemma":[0.00001397481,0.00005601708,0.0008269205,0.000005137464,0.000005415051,0.0001515447,0.00000953554,0.001604112,0.9949719,0.00008092426,0.002267503,0.000007073336],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9111241,0.0007765186,0.08244586,0.0002646367,0.00007551365,0.0001779838,0.0003690073,0.0007101137,0.004056412],"genre_scores_gemma":[0.8963757,0.000829308,0.09674345,0.0001366358,0.00001943644,0.0002591929,0.0003441668,0.0002364889,0.005055722],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001199592,"threshold_uncertainty_score":0.00310868,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01058568223280123,"score_gpt":0.240882479313232,"score_spread":0.2302967970804307,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}