{"id":"W3194469425","doi":"10.1101/2021.08.22.457263","title":"Reconstitution of surface lipoprotein translocation reveals Slam as an outer membrane translocon in Gram-negative bacteria","year":2021,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Canadian Institutes of Health Research; National Institutes of Health; Stockholms Universitet","keywords":"Periplasmic space; Bacterial outer membrane; Translocon; Chromosomal translocation; Biology; Bacteria; Cell biology; Membrane; Inner membrane; Microbiology; Escherichia coli; Biophysics; Biochemistry; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002670888,0.0004047625,0.0002786195,0.0002445749,0.0001741826,0.000430492,0.0003252159,0.000413769,0.000531456],"category_scores_gemma":[0.0002406458,0.000180225,0.0002887036,0.000240837,0.0003176802,0.0002218968,0.000471802,0.0005372536,0.0004834586],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006074217,"about_ca_system_score_gemma":0.0002532042,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001189133,"about_ca_topic_score_gemma":0.0007704073,"domain_scores_codex":[0.9998289,0.00004506942,0.00001780446,0.00002559636,0.00004758863,0.00003503505],"domain_scores_gemma":[0.9998726,0.00003034197,0.00003144579,0.00002330263,0.00001210886,0.00003017357],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000116396,0.00003206994,0.0001479781,0.00003070381,0.000004542238,0.00006437229,0.00002637257,0.0001942654,0.998744,0.0002114936,0.00002460056,0.0004031238],"study_design_scores_gemma":[0.00001850503,0.00009239147,0.001109257,0.000006793883,0.000006208194,0.00008752973,0.0000394931,0.002588965,0.9948394,0.00007953569,0.001125556,0.000006512481],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9958806,0.0002924647,0.002969074,0.0001009494,0.00001917912,0.00001623956,0.0002849146,0.00007997928,0.00035659],"genre_scores_gemma":[0.994214,0.0001989534,0.003309528,0.00002476243,0.00000377589,0.00001827658,0.0007765878,0.00005001157,0.001404043],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001189133,"threshold_uncertainty_score":0.004407108,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01091191647179515,"score_gpt":0.2239087562189436,"score_spread":0.2129968397471485,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}