{"id":"W3195654192","doi":"10.1021/acschembio.1c00445","title":"Identification of Ubiquitin Variants That Inhibit the E2 Ubiquitin Conjugating Enzyme, Ube2k","year":2021,"lang":"en","type":"article","venue":"ACS Chemical Biology","topic":"Ubiquitin and proteasome pathways","field":"Biochemistry, Genetics and Molecular Biology","cited_by":22,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Health Research Council of New Zealand; Royal Society Te Apārangi","keywords":"Ubiquitin; Deubiquitinating enzyme; Ubiquitin-conjugating enzyme; Allosteric regulation; Enzyme; Ubiquitin-Protein Ligases; Biochemistry; Ubiquitin ligase; Cell biology; Biology; Ubiquitins; Chemistry; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005081288,0.0001951088,0.0002551878,0.00002413192,0.00009147582,0.00002438253,0.0003726382,0.0003701306,0.0001089705],"category_scores_gemma":[0.0008145453,0.0001572561,0.0001257,0.0001525244,0.0003120427,0.000005291896,0.0003743152,0.0002126045,0.00002802604],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001318194,"about_ca_system_score_gemma":0.0001155619,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001175533,"about_ca_topic_score_gemma":0.000006758878,"domain_scores_codex":[0.9983025,0.0002315005,0.0004807104,0.0005100445,0.0001176251,0.0003576416],"domain_scores_gemma":[0.9987444,0.0001317376,0.0002766964,0.0005680136,0.0002053691,0.00007376655],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003828769,0.00005839375,0.0009798494,0.00003084864,0.00005095925,0.000002986882,0.00007604995,0.000001015897,0.9928084,0.002308948,0.0006032117,0.003041048],"study_design_scores_gemma":[0.0004178433,0.00006918378,0.0002064766,0.0000206711,0.00002350158,0.00005285505,0.000145966,0.00001827083,0.9906769,0.001343139,0.006841711,0.0001834963],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9948171,0.001230262,0.0006422476,0.0008307548,0.0001705891,0.0001984579,0.000116629,0.00002071519,0.001973282],"genre_scores_gemma":[0.9973974,0.0001719178,0.0002589236,0.0009221128,0.0003159544,0.00005303728,0.0005608469,0.00002279331,0.0002970434],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0062385,"threshold_uncertainty_score":0.6412718,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01858354100647068,"score_gpt":0.2622772829363064,"score_spread":0.2436937419298358,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}